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Merck
CN

56759

Atto 425

BioReagent, suitable for fluorescence

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About This Item

Empirical Formula (Hill Notation):
C22H27NO6
CAS Number:
652966-03-5
Molecular Weight:
401.45
MDL number:
MFCD32670704
UNSPSC Code:
12352108
PubChem Substance ID:
329758237
NACRES:
NA.32

Key Documents

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Product Name

Atto 425, BioReagent, suitable for fluorescence

SMILES string

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(O)=O)c3cc2OC1=O

InChI

1S/C22H27NO6/c1-5-28-20(26)16-10-14-9-15-13(2)12-22(3,4)23(8-6-7-19(24)25)17(15)11-18(14)29-21(16)27/h9-11,13H,5-8,12H2,1-4H3,(H,24,25)

InChI key

WNDDWSAHNYBXKY-UHFFFAOYSA-N

product line

BioReagent

assay

≥90% (HPLC)

form

(Report result)

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm, >90%
425 nm, >90%

fluorescence

λex 440 nm; λem 484 nm

λ

in PBS, pH 7.4
 in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 423-429 nm Amax

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 is suitable for preparation of fluorescence labeling reagents.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9643
BCCN7151
BCCN5822
BCCN4646
BCCM0690

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Katrin G Heinze et al.
Biophysical journal, 86(1 Pt 1), 506-516 (2003-12-26)
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence
Triple FRET: a tool for studying long-range molecular interactions.
Elke Haustein et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 745-748 (2003-08-07)
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
Benzothiazole dye thioflavin T (ThT) is a sensitive probe for amyloid fibril detection. The ThT probing is based on its unique ability to form highly fluorescent complexes with amyloid and amyloid-like fibrils. In this work we propose an approach of
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 115(39), 11519-11524 (2011-08-26)
The fluorescence of the benzothiazole dye thioflavin T (ThT) is a well-known test for amyloid fibril formation. It has now become evident that ThT can also be used for structural investigations of amyloid fibrils and even for the treatment of
Richard S Agnes et al.
Journal of the American Chemical Society, 132(17), 6075-6080 (2010-04-13)
A fluorescent sensor of protein kinase activity has been developed and used to characterize the compartmentalized location of cAMP-dependent protein kinase activity in mitochondria. The sensor functions via a phosphorylation-induced release of a quencher from a peptide-based substrate, producing a
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