Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
All Photos(1)

Key Documents

View All Documentation

56496

Sigma-Aldrich

Calcein-AM

Small Package (20 X 50 μg ), ≥90.0% (HPLC)

Synonym(s):

Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester, fluorescein complexone, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester

Sign Into View Organizational & Contract Pricing

Select a Size

10 MG
¥1,617.22
25 MG
¥2,669.81
100 MG
¥8,019.88

¥1,617.22

Please contact Customer Service for Availability
New, lower price on this item!
Request a Bulk Order
All Photos(1)

Select a Size

Change View
10 MG
¥1,617.22
25 MG
¥2,669.81
100 MG
¥8,019.88

About This Item

Empirical Formula (Hill Notation):
C46H46N2O23
CAS Number:
148504-34-1
Molecular Weight:
994.86
MDL number:
MFCD05861516
UNSPSC Code:
12171500
PubChem Substance ID:
329758218
NACRES:
NA.32

¥1,617.22

Please contact Customer Service for Availability
New, lower price on this item!
Request a Bulk Order

Assay

≥90.0% (HPLC)

fluorescence

λex 496 nm; λem 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca2+

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3

InChI key

XKFSBWQWNMZWFA-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MMHVMFX24MXRP2USBCMMQUANK01
Milliflex® Rapid System 2.0 for use with Milliflex Oasis®, suitable for bioburden testing, sterility testing

MXRDP2KT01

Milliflex® Rapid System 2.0

Milliflex Oasis® Filtration Unit 0.45 μm PVDF Durapore® membrane with 100 mL funnel, designed for Milliflex® Rapid 2.0

MMHVMFX24

Milliflex Oasis® Filtration Unit

Milliflex® Rapid 2.0 Detection Tower – USB cable

MXRP2USBC

Milliflex® Rapid 2.0 Detection Tower – USB cable

Milliflex® Quantum Rapid Detection System Supplied with 1 Reader, 1 Reader Stand, 1 Membrane Transfer Tool, 1 Removal Rack, 1 Camera and Installation flash drive

MMQUANK01

Milliflex® Quantum Rapid Detection System

AC/DC input

100 V / 230 V, 50-60 Hz

AC/DC input

-

AC/DC input

-

AC/DC input

100 V / 200 V, 50-60 Hz

packaging

pkg of 1 kit

packaging

pkg of 24 units

packaging

pkg of 1 unit

packaging

-

agency

EP 2.6.1

agency

-

agency

-

agency

-

product line

Milliflex®

product line

-

product line

-

product line

-

parameter

10 kg operating weight (22 lb) (Autospray Station)

parameter

100 mL sample volume

parameter

-

parameter

4.4 kg operating weight, 45 °C temperature (for samples)

General description

Calcein-AM, also known as Calcein-acetoxymethyl ester, is a non-fluorescent molecule converted into an anionic fluorescent form by intracellular esterase enzymes. It provides a continuous visualization of adherent cells during the experiment.[1] Calcein-AM is a vital dye that introduces Calcein in living cells with intact cell membranes. The fluorescent part of Calcein-AM localizes in the intracellular spaces after esterase-dependent cellular trapping, displaying cytotoxic activity in cells.[2]

Application

Calcein-AM is used as a cell viability stain and as a neutral substrate for multidrug (MDR) efflux transporters. In cancer research, it is used as a neutral substrate for multidrug resistance protein MRP. Fluorescence assays to determine human erythrocyte viability and aging uses Calcein-AM as a probe.[3]
Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCM9056
    BCCK9243
    BCCH5457
    BCCG9095
    BCCD6943

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    B Jonsson et al.
    European journal of cancer (Oxford, England : 1990), 32A(5), 883-887 (1996-05-01)
    The aim of this study was to determine the in vitro cytotoxicity of calcein acetoxymethyl ester (Calcein/AM) on primary cultures derived from solid and haematological human tumours. Calcein/AM is a fluorescent dye that localises intracellularly after esterase-dependent cellular trapping and
    Daniela Bratosin et al.
    Cytometry. Part A : the journal of the International Society for Analytical Cytology, 66(1), 78-84 (2005-05-26)
    A highly sensitive, fast, and simple flow cytometric assay to assess human red blood cell (RBCs) viability and aging is reported. The assay described in this report is based on the use of acetoxymethyl ester of calcein (calcein-AM), a fluorescein
    Jacopo Uggeri et al.
    Histochemistry and cell biology, 122(5), 499-505 (2004-10-27)
    Calcein-acetoxymethylester (calcein-AM) is a non-fluorescent, cell permeant compound, which is converted by intracellular esterases into calcein, an anionic fluorescent form. It is used in microscopy and fluorometry and provides both morphological and functional information of viable cells. In this study
    Xiao-Cong Huang et al.
    Biochemical pharmacology, 85(9), 1257-1268 (2013-02-19)
    High intrinsic or acquired expression of membrane spanning, adenosine triphosphate binding cassette (ABC) transporter proteins, such as P-glycoprotein (P-gp), in cancers represents a major impediment to chemotherapy, with accelerated drug efflux leading to multi-drug resistance (MDR). Although ABC transporter inhibitors
    Bao-Ling Du et al.
    Journal of biomedical materials research. Part A, 103(4), 1533-1545 (2014-07-22)
    Biological materials combined with genetically-modified neural stem cells (NSCs) are candidate therapy targeting spinal cord injury (SCI). Based on our previous studies, here we performed gelatin sponge (GS) scaffold seeded with neurotrophin-3 (NT-3) and its receptor TrkC gene modifying NSCs
    View All Related Papers

    Articles

    Detect, Visualize and Quantify Single Post-Translational Modifications

    Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service