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540-05A

Sigma-Aldrich

Human Lung Microvascular Endothelial Cells: HLMVEC, adult

Synonym(s):

HLMVEC, HLMVEC cells

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human lung (normal)

Quality Level

100

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

Endothelial

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

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The pulmonary vasculature is of great physiological/pathological significance. It is now recognized to be a tissue composed of metabolically active, functionally responsive cells, that interact with circulating substrates and regulate the composition of systemic arterial blood, affect target organ functions, and contribute to thrombosis, hemostasis and immune reactions, as well as tumor metastasis. Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro.

HLMVEC have been used to elucidate the therapeutic effects of Angiotensin I-converting enzyme (ACE) inhibitors, and the results revealed that they provide an additional benefit to patients by activating bradykinin B1 receptor leading to prolonged nitric oxide (NO) production in endothelial cells and inhibition of PKCε (Ignjatovic, 2004; Stanisavljevic, 2006).

Cell Line Origin

Lungs

Application

lung physiology, metabolism, regulate blood composition, hemostasis, immune reactions, therapeutic effects, leukocyte migration,

Components

Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 3rd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 15 doublings

Subculture Routine

Please refer to the HLMVEC Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

高风险级别生物产品--人源产品

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Huiyuan Yan et al.
Experimental and therapeutic medicine, 26(1), 319-319 (2023-06-05)
Acute lung injury (ALI) is associated with increased lung inflammation and lung permeability. The present study aimed to determine the role of inactive rhomboid-like protein 2 (iRHOM2) in ALI in lipopolysaccharide (LPS)-induced pulmonary microvascular endothelial cell model. Human pulmonary microvascular

Protocols

Human Lung Microvascular Endothelial Cells (HLMVEC) Culture Protocol

Technical information for working with human lung microvascular endothelial cells including thawing, subculturing and cryopreservation

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