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Merck
CN

51448C

Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12

suitable for cell culture

Synonym(s):

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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description

for research or for further manufacturing use
with 3151 mg/L dextrose, with 2.5 mM L-glutamine, with 55 mg/L sodium pyruvate

Quality Level

500

sterility

sterile

form

liquid

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

components

phenol red: yes
L-glutamine: yes
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

DMEM/F12 was originally developed to study hormonal requirements of cells in culture.

Application

Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 has been used a primary culture media for propagation of adipose-derived mesenchymal stem cells.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
0000416320
0000416320
0000352560
0000352560
0000346305

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The effect of metamizole and tolfenamic acid on canine and equine adipose-derived mesenchymal stem cells (ASCs) an in vitro research.
Nicpon J, et al.
Polish Journal of Veterinary Sciences, 18(1), 3-11 (2015)
Richard L Hornsby et al.
Scientific reports, 10(1), 9620-9620 (2020-06-17)
The causative agent of leptospirosis includes multiple serovars and species of pathogenic leptospires that are excreted via urine from reservoir hosts of infection. Primary isolation takes weeks to months, and is limited to semi-solid media at 28-30 °C. Here we present
Tobias M Lindig et al.
Histochemistry and cell biology, 131(1), 1-12 (2008-09-23)
We have compared the three-dimensional (3D) morphology of stubby and spiny neurons derived from the human small intestine. After immunohistochemical triple staining for leu-enkephalin (ENK), vasoactive intestinal peptide (VIP) and neurofilament (NF), neurons were selected and scanned based on their

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