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Merck
CN

51412C

Minimum Essential Medium

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Minimum Essential Medium Eagle, MEM

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

Key Documents

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Product Name

Minimum Essential Medium, with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

components

L-glutamine: no
NaHCO3: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes

storage temp.

2-8°C

Quality Level

500

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Application

Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons.

General description

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO2 gas.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
0000486948
0000486948
0000411539
0000411539
0000381769

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