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biological source
human lung (normal parenchyma)
Quality Level
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 46
morphology
fibroblast
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
asthma; tuberculosis; cardiovascular diseases; cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
HLF provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.
HLF have been utilized in a number of research publications, for example to:
HLF provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.
HLF have been utilized in a number of research publications, for example to:
- Demonstrate differential gene expression patterns between fetal and adult lung fibroblasts, in particular showing that fetal fibroblasts express higher level of TGF-β receptor interacting protein 1 (TRIP-1) and consequently are less able to contract collagen than adult lung fibroblasts, leading to better healing of acute injuries in very immature lungs compared to higher levels of fibrosis observed in older patients (Navarro, 2009), and additionally discover that TRIP-1 regulates lung fibroblast/myofibroblast trans-differentiation (Nyp, 2014)
- Show that angiotensin II promotes lung fibrosis by activating expression of IL-4, IL-7, and PDGF-D increasing the extracellular matrix formation, and that this process can be blocked by angiotensin receptor inhibitor candesartan (Okada, 2009)
- Determine that phosphorylation of C/EBPβ contributes to lung fibrosis (Buck, 2011)
- Demonstrate that increased tissue rigidity activates toll-like receptor 2 and NF-κB in lung fibroblasts and induces expression of cytokines, CXCL1-3, IL-8 and TNF-α (Zheng, 2011)
- Demonstrate that 2-ethoxyestradiol, a non-estrogenic estradiol metabolite analog, inhibits cell growth and retards the progression of pulmonary fibrosis and hypertension (Tofovic, 2008, 2009)
- Identify the hypoxia-induced ROS/RhoA–ROCK dependent actin reorganization as a mediator of myofibroblast transformation and collagen synthesis in lung fibrosis (Ni, 2013)
Cell Line Origin
Lungs
Application
pulmonary function, gene expression, healing and repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development
Components
Basal Medium containing 10% FBS & 10% DMSO
Preparation Note
- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Subculture Routine
Please refer to the HLF Culture Protocol.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
新产品
Certificates of Analysis (COA)
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Protocols
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
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