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47860

Sigma-Aldrich

Basic Fuchsin

for microscopy (Bact., Bot., Hist.), indicator (pH 1.0-3.1)

Synonym(s):

Basic Violet 14, Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2-methyl hydrochloride, Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2-methyl

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About This Item

Empirical Formula (Hill Notation):
C20H20ClN3
CAS Number:
632-99-5
Molecular Weight:
337.85
Colour Index Number:
42510
UNSPSC Code:
12171500
PubChem Substance ID:
24871527
NACRES:
NA.47

grade

for microscopy (Bact., Bot., Hist.)
indicator (pH 1.0-3.1)

application(s)

environmental

SMILES string

NC1=CC=C(C(C2=CC(C)=C(N)C=C2)=C3C=CC(C=C3)=N)C=C1.[Cl]

InChI

1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,23H,21-22H2,1H3;1H

InChI key

AXDJCCTWPBKUKL-UHFFFAOYSA-N

General description

Basic fuchsin, a triphenylmethane dye is commonly a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II (no CI number) and new fuchsine (CI 42520). Basic fuchsin is widely used in tissue-specific staining of biological and coloring several materials including orlon, cotton, leather, paper etc. It is also used to stain collagen, muscle and mitochondria.

Application

Basic Fuchsin has been used in several biological tests including:
  • Acid-fast staining with carbol-fuchsine
  • Periodic Acid-Schiff method
  • Feulgen technique for DNA staining
  • ENDO culture medium for distinguishing between the bacteria Escherichia coli and Aerobacter aerogenes
  • Leifson′s method for bacterial flagella staining.

Biochem/physiol Actions

Basic fuchsin possesses anesthetic, bactericidal (gram-positive), and fungicidal properties.

Analysis Note

homologous mixture of dyes, contains dominantly (~35-40%) Basic Fuchsin (C.I. 42510)

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H351

Precautionary Statements

P201 - P308 + P313

Hazard Classifications

Carc. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Daria Bloch et al.
Development (Cambridge, England), 146(17) (2019-08-11)
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