All Photos(1)

Documents

47614

Fluram

Name: Fluram
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥99.0% (UV)

Synonym(s):

Fluorescamine, 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione

Sign Into View Organizational & Contract Pricing

All Photos(1)

About This Item

Empirical Formula (Hill Notation):

C₁₇H₁₀O₄

CAS Number:

38183-12-9

Molecular Weight:

278.26

Beilstein:

921143

EC Number:

253-814-5

MDL number:

MFCD00005928

UNSPSC Code:

12352200

PubChem Substance ID:

329757329

product line

BioReagent

Assay

≥99.0% (UV)

mp

153-157 °C (lit.)
153-157 °C

solubility

acetonitrile: soluble
ethanol: soluble

fluorescence

λ_ex 234 nm
λ_ex 390 nm; λ_em 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)

suitability

suitable for fluorescence

SMILES string

O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14

InChI

1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H

InChI key

ZFKJVJIDPQDDFY-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Non-enzymatic cleavage of Hsp90 by oxidative stress leads to actin aggregate formation: A novel gain-of-function mechanism.

José Pedro Castro et al.

Redox biology, 21, 101108-101108 (2019-01-21)

Aging is accompanied by the accumulation of oxidized proteins. To remove them, cells employ the proteasomal and autophagy-lysosomal systems; however, if the clearance rate is inferior to its formation, protein aggregates form as a hallmark of proteostasis loss. In cells

Simple and sensitive spectrofluorimetric method for the determination of oseltamivir phosphate in capsules through derivatization with fluorescamine.

Zeynep Aydoğmuş

Journal of fluorescence, 19(4), 673-679 (2009-02-03)

A new, simple and sensitive spectrofluorimetric method has been developed for the determination of oseltamivir phosphate (OSP) in capsules. The method is based on the reaction between oseltamivir and fluorescamine in borate buffer solution of pH 8.50 to give highly

Prefluorescent nitroxide probe for the highly sensitive determination of peroxyl and other radical oxidants.

Min Jia et al.

Analytical chemistry, 81(19), 8033-8040 (2009-10-01)

Fluorescamine derivatized 3-amino-2,2,5,5,-tetramethyl-1-pyrrolidinyloxy (I) is shown to undergo an irreversible reaction with peroxyl radicals and other radical oxidants to generate a more highly fluorescent diamagnetic product (II) and thus can be used as a highly sensitive and versatile probe to

Controlling the adhesion and differentiation of mesenchymal stem cells using hyaluronic acid-based, doubly crosslinked networks.

Amit K Jha et al.

Biomaterials, 32(10), 2466-2478 (2011-01-11)

We have created hyaluronic acid (HA)-based, cell-adhesive hydrogels that direct the initial attachment and the subsequent differentiation of human mesenchymal stem cells (MSCs) into pre-osteoblasts without osteogenic supplements. HA-based hydrogel particles (HGPs) with an average diameter of 5-6 μm containing

Biodegradable poly(ethylene glycol) hydrogels based on a self-elimination degradation mechanism.

Manjeet Deshmukh et al.

Biomaterials, 31(26), 6675-6684 (2010-06-22)

Two vinyl sulfone functionalized crosslinkers were developed for the purpose of preparing degradable poly(ethylene glycol) (PEG) hydrogels (EMXL and GABA-EMXL hydrogels). A self-elimination degradation mechanism in which an N-terminal residue of a glutamine is converted to pyroglutamic acid with subsequent

View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service