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47608

Sigma-Aldrich

Formaldehyde solution

for molecular biology, BioReagent, ≥36.0% in H2O (T)

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Synonym(s):
Formic aldehyde, Formol, Formalin
Linear Formula:
HCHO
CAS Number:
50-00-0
Molecular Weight:
30.03
Beilstein:
1209228
MDL number:
MFCD00003274
UNSPSC Code:
12352114
PubChem Substance ID:
329757328
NACRES:
NA.52

grade

for molecular biology

Quality Level

100

vapor density

1.03 (vs air)

vapor pressure

52 mmHg ( 37 °C)

product line

BioReagent

form

liquid

autoignition temp.

572 °F

contains

~10% methanol as stabilizer

concentration

≥36.0% in H2O (T)
36.0-38.0% (aldehyde method)

technique(s)

electrophoresis: suitable

impurities

≤0.03% free acid (as HCOOH)

ign. residue

≤0.05% (as SO4)

pH

2.8-4.0

density

1.09 g/mL at 20 °C
1.09 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤20 mg/kg

cation traces

Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Na: ≤100 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Zn: ≤1 mg/mL

λ

2 M in H2O

UV absorption

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

suitability

in accordance for electrophoresis of RNA in agarose gels

storage temp.

room temp

SMILES string

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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General description

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.

Application

Formaldehyde solution is suitable for:
  • denaturation and separation of RNA molecules
  • anti-bacterial treatment
  • fixation of cells
Formaldehydesolution has been used:
  • in the fixation of human spermatogonial stem cell transplantation (SSCT).
  • to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.
  • in fixing zebra fish larvae for neutrophil staining.

Caution

may contain precipitate (paraformaldehyde)

Principle

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

Target Organs

Eyes,Central nervous system, Respiratory system

WGK

WGK 3

Flash Point(F)

closed cup

Flash Point(C)

closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation
Onofre J, et al.
Reproductive Biomedicine Online, 40(2), 261-269 (2020)
Formaldehyde
Gerberich, H R and Seaman, G C.
Kirk-Othmer Encyclopedia of Chemical Technology (2013)
Jin Qian et al.
Virology, 474, 10-18 (2014-12-03)
Interferon-induced transmembrane (IFITM) proteins inhibit the entry of a large number of viruses. Not surprisingly, many viruses are refractory to this inhibition. In this study, we report that different strains of HIV and SIV are inhibited by human IFITM proteins
Dannis G van Vuurden et al.
Neuro-oncology, 16(7), 946-959 (2014-01-29)
Glial brain tumors cause considerable mortality and morbidity in children and adults. Innovative targets for therapy are needed to improve survival and reduce long-term sequelae. The aim of this study was to find a candidate tumor-promoting protein, abundantly expressed in
Oliver Wicht et al.
Journal of virology, 88(14), 7952-7961 (2014-05-09)
Isolation of porcine epidemic diarrhea coronavirus (PEDV) from clinical material in cell culture requires supplementation of trypsin. This may relate to the confinement of PEDV natural infection to the protease-rich small intestine of pigs. Our study focused on the role
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