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47190

Sigma-Aldrich

5-Fluoroorotic acid hydrate

≥99.0% (TLC)

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Synonym(s):
2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid, 5-Fluorouracil-4-carboxylic acid
Empirical Formula (Hill Notation):
C5H3FN2O4 · xH2O
CAS Number:
207291-81-4
Molecular Weight:
174.09 (anhydrous basis)
EC Number:
211-876-0
MDL number:
MFCD00150658
UNSPSC Code:
12352204
PubChem Substance ID:
57650902
NACRES:
NA.25

Quality Level

100

Assay

≥99.0% (TLC)

form

solid

mp

278 °C (dec.) (lit.)

solubility

4 M NH4OH: 50 mg/mL, clear, faintly yellow

storage temp.

−20°C

SMILES string

OC(C(NC(N1)=O)=C(F)C1=O)=O.[H]O[H]

InChI

1S/C5H3FN2O4.H2O/c6-1-2(4(10)11)7-5(12)8-3(1)9;/h(H,10,11)(H2,7,8,9,12);1H2

InChI key

LODRRYMGPWQCTR-UHFFFAOYSA-N

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Application

Useful in the selection of orotidine-5′-phosphate decarboxylase mutants of Saccharomyces cerevisiae.

Other Notes

Selective agent in yeast molecular genetics

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302,H315,H319,H335

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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J D Boeke et al.
Methods in enzymology, 154, 164-175 (1987-01-01)
5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on

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