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Merck
CN

44670

Fast Blue BB Salt hemi(zinc chloride) salt

suitable for microscopy

Synonym(s):

4-Amino-2,5-diethoxybenzanilide diazotated zinc double salt, 4-Benzoylamino-2,5-diethoxybenzenediazonium chloride hemi(zinc chloride) salt

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About This Item

Linear Formula:
C17H18N3O3Cl · 1/2 ZnCl2
CAS Number:
5486-84-0
Molecular Weight:
415.94
EC Number:
226-817-4
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57650235
MDL number:
MFCD00074765
Colour Index Number:
37175
Beilstein/REAXYS Number:
1845288

Key Documents

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Product Name

Fast Blue BB Salt hemi(zinc chloride) salt, suitable for microscopy

InChI key

CMFRFQODFZBKTI-UHFFFAOYSA-L

InChI

1S/2C17H17N3O3.4ClH.Zn/c2*1-3-22-15-11-14(20-18)16(23-4-2)10-13(15)19-17(21)12-8-6-5-7-9-12;;;;;/h2*5-11H,3-4H2,1-2H3;4*1H;/q;;;;;;+2/p-2

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CCOc1cc([N+]#N)c(OCC)cc1NC(=O)c2ccccc2.CCOc3cc([N+]#N)c(OCC)cc3NC(=O)c4ccccc4

form

powder

technique(s)

microbe id | staining: suitable

mp

157 °C

solubility

hot water: 0.01 g/10 mL

εmax

≥100 at 390-400 nm in water
≥200 at 325-335 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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Application

Fast blue BB salt hemi(zinc chloride) salt has been used to prepare substrate solution for tissue-blot enzyme-immunoassay procedure. It has been used in immunohistochemical staining.

General description

Fast blue BB salt hemi(zinc chloride) salt is suitable as a common coloring agent for TLC (thin layer chromatography) analysis of cannabis. It is also suitable for histochemistry studies. It is of 415.9kDa.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM5845
BCCK8220
BCCH2833
BCCF7449
BCCC8930

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B Algermissen et al.
Experimental dermatology, 3(6), 290-297 (1994-12-01)
In order to gain insights into the dynamics of mast cell subpopulations in normal and diseased skin, a novel enzyme-histochemical double and triple staining method was employed that allowed the detection of metachromasia (toluidine blue) and the mast cell proteases
Marjorie B Medina
Journal of agricultural and food chemistry, 59(5), 1565-1571 (2011-02-12)
A new method for the detection of phenolics in food systems was developed. This method is based on interactions of phenolics with Fast Blue BB diazonium salt in alkali pH, forming azo complexes, with the absorbance measured at 420 nm
L Wagner et al.
Journal of immunological methods, 142(2), 147-155 (1991-09-13)
A trypsin-like serine esterase (SE) is known to be present in cultured cells with cytolytic potential. The distribution pattern of this enzyme in haematological cells and body tissues has been assessed using a method which permits rapid identification of individual
R B Hughes et al.
Journal of forensic sciences, 24(4), 842-846 (1979-10-01)
We are reporting what we believe to be a safe, rapid TLC system and spray for the identification of marihuana. The use of Fast Blue 2B salt greatly enhances the specificity of the TLC analysis of suspected marihuana samples.
T Fujimoto et al.
Histochemistry, 65(3), 217-222 (1980-01-01)
The correlated distribution of intramembranous particles and sulfhydryl groups was examined in normal adult rabbit erythrocyte ghosts. Ghosts were treated to exhibit characteristic patterns of particle distribution and sulfhydryl groups were stained with Fast Blue BBN. It was shown that
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