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4352135

Sigma-Aldrich

iTRAQ® Reagents Multiplex Kit

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Synonym(s):
isobaric tags for relative and absolute quantitation 
UNSPSC Code:
12352200
NACRES:
NA.24

Quality Level

shipped in

dry ice

storage temp.

−20°C

General description

Contains iTRAQ® reagents 114, 115, 116, 117, the appropriate buffers and reagents for ten 2-plex, six 3-plex, or five 4-plex assays. 10-PK trypsin is sold separately. Each individual reagent is capable of labeling up to 100 μg of protein.

Application

The iTRAQ® Reagents Multiplex Kit provides iTRAQ® reagents and protocol to label peptides after digestion. This widely used generic labeling kit can be used for virtually any workflow.
iTRAQ® Reagents Multiplex Kit has been used for the labeling of peptides for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.

Analysis Note

To view the general Protocol for iTRAQ Reagents, please visit this Protocol link.

To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

The Certificate of Analysis (CofA) for your specific lot is accessible by entering the product number and lot number at this CofA access link.

Legal Information

iTraq is a registered trademark of AB Sciex Pte. Ltd.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

WGK

WGK 3

Flash Point(F)

35.6 °F

Flash Point(C)

2.0 °C

Regulatory Information

危险化学品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zeiner CA, et al.
PLoS ONE, 11, e0157844-e0157844 (2016)
Xiuyan Huang et al.
Journal of proteome research, 9(12), 6759-6773 (2010-10-20)
Regulatory T cells (Treg) induce robust neuroprotection in murine models of neuroAIDS, in part, through eliciting anti-inflammatory responses for HIV-1-infected brain mononuclear phagocytes (MP; macrophage and microglia). Herein, using both murine and human primary cell cultures in proteomic and cell
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Translational psychiatry, 10(1), 144-144 (2020-05-14)
Major depressive disorder (MDD) is a leading cause of morbidity with a lifetime prevalence of 10%. There is increasing evidence suggesting synaptic dysfunction and impaired integrity of certain brain circuits in MDD. Here we investigate the cerebrospinal fluid proteome of
Stanislav S Rubakhin et al.
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Cell-to-cell signaling peptides play important roles in neurotransmission, neuromodulation, and hormonal signaling. Significant progress has been achieved in qualitative investigations of signaling peptides in the nervous system using single cell matrix-assisted laser desorption/ionization mass spectrometry. However, quantitative information about signaling

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