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Key Documents

38733

Sigma-Aldrich

Laemmli Lysis-buffer

non smelling

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50 EA
CN¥1,852.91

CN¥1,852.91


Estimated to ship onApril 07, 2025Details


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50 EA
CN¥1,852.91

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

CN¥1,852.91


Estimated to ship onApril 07, 2025Details


Request a Bulk Order

form

liquid

composition

bromophenol blue, 0.004%
DTT, 400 mM
glycerol, 20%
SDS, 4%
TRIS, 0.125 M

pH

~6.8

suitability

in accordance for electrophoresis test

storage temp.

−20°C

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1 of 4

This Item
BAH136800070WHA10370172WHA10370105
sterility

sterile

sterility

sterile

sterility

non-sterile

sterility

-

packaging

pack of 50 ea

packaging

pack of 50 ea, pkg of (supplied in a tray)

packaging

pkg of 100 ea

packaging

pkg of 100 ea

manufacturer/tradename

Bel-Art H13680-0040

manufacturer/tradename

Bel-Art H13680-0070

manufacturer/tradename

Whatman 10370172, Whatman Article No. 28414772 (US reference)

manufacturer/tradename

Whatman 10370105, Whatman Article No. 28414766 (US reference)

pore size

40 μm porosity

pore size

70 μm porosity

pore size

~3.0 μm (Grade GF 8)

pore size

~3.0 μm (Grade GF 8)

Application

Laemmli Lysis-buffer is an non-smelling lysis buffer used to prepare protein samples for SDS gel electrophoresis.

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the difference between the two Laemmli buffers S3401 and 38733?

    Product No. S3401 is 0.125 M Tris (pH 6.8) in 80% water/20% glycerol, with 4% SDS, 10% 2-mercaptoethanol and 0.004% bromophenol blue. 38733 is 0.125 M Tris in 80% water/20% glycerol, with 4% SDS, 400 mM DTT and 0.004% bromophenol blue.  So the only difference is the reducing agent; 2-mercaptoethanol (strong odor) vs. the DTT (not nearly as strong an odor).

  4. What is the advantage of using Laemmli Lysis-buffer, Product No. 38733?

    The odor of 38733 is not nearly as strong as that of S3401 (which has 2-mercaptoethanol).  Some people are very sensitive to the odor of 2-mercaptoethanol.

  5. Is Laemmli Lysis-buffer, Product No. 38733, a 1× or a 2× solution?

    For most applications, it is considered to be a 2X solution.

  6. Why do the Laemmli sample buffers like S3401 and 38733 have glycerol in them?

    An aqueous buffer with 20% glycerol is denser than one without. So when the sample is added to the well of an electrophoresis gel, it tends to stay in the well.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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