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38733

Sigma-Aldrich

Laemmli Lysis-buffer

non smelling

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

Quality Level

100

form

liquid

composition

bromophenol blue, 0.004%
DTT, 400 mM
glycerol, 20%
SDS, 4%
TRIS, 0.125 M

pH

~6.8

suitability

in accordance for electrophoresis test

storage temp.

−20°C

Related Categories

Application

Laemmli Lysis-buffer is an non-smelling lysis buffer used to prepare protein samples for SDS gel electrophoresis.

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H315,H318

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the difference between the two Laemmli buffers S3401 and 38733?

    Product No. S3401 is 0.125 M Tris (pH 6.8) in 80% water/20% glycerol, with 4% SDS, 10% 2-mercaptoethanol and 0.004% bromophenol blue. 38733 is 0.125 M Tris in 80% water/20% glycerol, with 4% SDS, 400 mM DTT and 0.004% bromophenol blue.  So the only difference is the reducing agent; 2-mercaptoethanol (strong odor) vs. the DTT (not nearly as strong an odor).

  4. What is the advantage of using Laemmli Lysis-buffer, Product No. 38733?

    The odor of 38733 is not nearly as strong as that of S3401 (which has 2-mercaptoethanol).  Some people are very sensitive to the odor of 2-mercaptoethanol.

  5. Is Laemmli Lysis-buffer, Product No. 38733, a 1× or a 2× solution?

    For most applications, it is considered to be a 2X solution.

  6. Why do the Laemmli sample buffers like S3401 and 38733 have glycerol in them?

    An aqueous buffer with 20% glycerol is denser than one without. So when the sample is added to the well of an electrophoresis gel, it tends to stay in the well.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Sean R Gallagher
Current protocols in protein science, Chapter 10, Unit 10-Unit 10 (2012-04-04)
One-dimensional gel electrophoresis of proteins provides information about the molecular size, amount, and purity of a protein sample. Separated proteins can be recovered from polyacrylamide gels for subsequent characterization by a variety of secondary techniques, such as mass spectrometry to
Xiaoqiang Wu et al.
Analytical biochemistry, 421(1), 347-349 (2011-11-01)
A modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol is described. The new method saves 30 min for gel casting without loss of the resolution power of Laemmli gel. In this method, both the upper and lower gels can
Vincent Archambault et al.
Genes & development, 22(19), 2707-2720 (2008-10-04)
The conserved Polo kinase controls multiple events in mitosis and cytokinesis. Although Polo-like kinases are regulated by phosphorylation and proteolysis, control of subcellular localization plays a major role in coordinating their mitotic functions. This is achieved largely by the Polo-Box
Sean R Gallagher
Current protocols in immunology, Chapter 8, Unit 8-Unit 8 (2008-04-25)
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to investigate subunit compositions, and to verify homogeneity of protein samples. It can also serve to purify proteins for use in further
Lisha Mou et al.
PloS one, 10(7), e0133997-e0133997 (2015-07-25)
Secretory azoospermia is a severe form of male infertility caused by unknown factors. DAX-1 is predominantly expressed in mammalian reproductive tissues and plays an important role in spermatogenesis because Dax-1 knockout male mice show spermatogenesis defects. To examine whether DAX-1
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