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356M-1

Sigma-Aldrich

Cytokeratin 5 & 6 (D5 & 16B4) Mouse Monoclonal Antibody

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UNSPSC Code:
12352200
NACRES:
NA.41

biological source

mouse

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

D5 & 16B4, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (356M-14)
vial of 0.5 mL concentrate (356M-15)
bottle of 1.0 mL predilute (356M-17)
vial of 1.0 mL concentrate (356M-16)
bottle of 7.0 mL predilute (356M-18)

manufacturer/tradename

Cell Marque™

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200

isotype

IgG1

control

mesothelioma

shipped in

wet ice

storage temp.

2-8°C

visualization

cytoplasmic

Gene Information

human ... KRT5(3852)

Related Categories

General description

Anti-CK 5 & 6 positivity is seen in 65% to 100% of epithelioid mesotheliomas, in 0% to 19% of lung adenocarcinomas, and in 22% to 80% of serous carcinoma of the ovary and peritoneum. Keratin 5 & 6 expression in lung adenocarcinoma is not common, and when it occurs, the reaction is usually confined to small focal areas or scattered cells. The most probable cause of this finding is the presence of squamous differentiation of lung adenocarcinoma. Anti-CK 5 & 6 positivity has been useful in recognizing squamous cell carcinoma of the skin. Less than 10% of carcinomas of the breast, colon, and prostate stain positively for this marker. Anti-CK 5 & 6 has also been used successfully as a myoepithelial cell marker in the prostate and breast to determine malignancy. Anti-CK 5 & 6 is a useful marker to distinguish lung squamous cell carcinoma from lung adenocarcinoma and large cell carcinoma within a panel including antibodies against TTF-1, Napsin A, p63, SOX-2, desmocollin3, and desmoglein3.

Quality


IVD
IVD
IVD
RUO

Linkage

Cytokeratin 5 & 6 Positive Control Slides, Product No. 356S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Neil A Abrahams et al.
American journal of clinical pathology, 120(3), 368-376 (2003-09-25)
We evaluated the sensitivity and specificity of cytokeratin (CK) 5/6 for distinguishing foci of atrophy from prostatic adenocarcinoma with and without previous hormonal adjuvant therapy and observed the intensity and pattern of staining in mimickers of prostatic adenocarcinoma (basal cell
Magali Lacroix-Triki et al.
Virchows Archiv : an international journal of pathology, 442(6), 548-554 (2003-04-25)
Previous studies have shown that basal-type cytokeratins (CKs) can distinguish usual ductal hyperplasia (UDH) from the spectrum of atypical ductal hyperplasia (ADH), ductal carcinoma in situ (DCIS) and lobular carcinoma in situ (LCIS). Indeed, expression of these CKs is weak
F Otterbach et al.
Histopathology, 37(3), 232-240 (2000-09-06)
This study was performed to determine the diagnostic value of keratin 5/6 (CK 5/6) immunophenotyping on routinely processed breast tissues. Six hundred and ninety-nine breast lesions, including normal tissues as well as benign and malignant lesions in 321 formalin-fixed, paraffin-embedded
Jorge S Reis-Filho et al.
Virchows Archiv : an international journal of pathology, 443(2), 122-132 (2003-07-29)
p63, cytokeratin (CK) 5/6 and CK 14 have been employed in diagnostic pathology as markers of basal, squamous and myoepithelial differentiation in several types of human neoplasms; however, there is scant data on the concurrent expression of these markers in
Peiguo G Chu et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 15(1), 6-10 (2002-01-18)
Cytokeratin 5/6 (CK 5/6) immunoreactivity has been observed in the vast majority of cases of malignant mesothelioma but only rarely in pulmonary adenocarcinomas. Thus, CK 5/6 has been used to distinguish malignant mesothelioma from pulmonary adenocarcinoma. However, the utility of
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