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Human Aortic Smooth Muscle Cells: HAOSMC, fetal

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human aorta (normal, tunica intima and tunica media, fibrous plaque-free)

Quality Level

100

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

smooth muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Human Aortic Smooth Muscle Cells (HAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.

HAOSMC have been utilized in dozens of research studies, for example, to:elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Liu, 2009; Tan, 2009; Seymour, 2010; Chen, 2011; Hirase, 2013; Stein, 2013);
  • understand hyperglycemia-related risk factors for atherosclerosis in diabetes patients (Maier, 2010), as well as effects of ethanol on vascular calcification (Oros, 2012);
  • investigate extracellular elastic matrix deposition and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Gacchina, 2010, 2011; Venkataraman, 2012; Phillippi, 2013);
  • study mechanisms and effects of mechanoregulation on proliferation and function of smooth muscle cells (Jiang, 2009; Venkataraman, 2013, 2014), and develop advanced stent technology, including novel surface materials, and drug and gene delivery systems to prevent restenosis and other occlusive vasculopathies (Brito, 2010; Deshpande, 2013; Kakade, 2013; Lamichhane, 2013).

Characterization: Positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Aorta

Application

aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine

Please refer to the HAOSMC Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Protocols

Culturing Human Aortic Smooth Muscle Cells

Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service