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Merck
CN

32857

Wright Stain solution

according to Wright

Synonym(s):

Eosin Methylene blue solution according to Wright, Wright’s eosin methylene blue

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About This Item

NACRES:
MA.02
PubChem Substance ID:
329754363
UNSPSC Code:
12171500
MDL number:
MFCD00005038

Key Documents

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Product Name

Wright Stain solution, according to Wright

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

form

liquid

quality

according to Wright

impurities

methanol

density

0.8 g/cm3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Wright stain solution was used during the exudate analysis of white blood cell concentration.

General description

Wright′s stain solution is commercially available and is generally used in hematology. The procedure of making it includes heating methylene blue with sodium bicarbonate, followed by combining the mixture with eosin. It is an alkaline solution; hence its concentration is continuously changing, owing to the progressive oxidizing action of the alkali, therefore Giemsa′s stain is added to preserve the azurophilic properties of the polychrome dyes.

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Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

51.8 °F

flash_point_c

11 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN7306
BCCM6752
BCCH2127
BCCG5787
BCCH9873

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A simplified Wright's stain technique for routine avian blood smear staining.
Shen PF and Patterson LT
Poultry Science, 62 (5), 923-924 (1983)
Biocompatibility and biofouling of MEMS drug delivery devices.
Biomaterials, 24 (11), 1959-1967 (2003)
Qian Kong et al.
Microvascular research, 130, 104009-104009 (2020-04-26)
The purpose of the present study was to investigate the possible role of TIPE2 on acute lung injury (ALI) induced by myocardial ischemia/reperfusion (MIR) in diabetic rats. Sprague-Dawley (SD) rats were randomly separated into four groups: control+sham (C + sham); control+MIR (C + MIR);
Young-Joo Jeong et al.
Anatomy & cell biology, 43(4), 294-302 (2011-01-27)
Asthma is a Th2-dependent disease mediated by IgE and Th2 cytokines, and asthmatic patients suffer from oxidative stresses from abnormal airway inflammation. Vitamin C is a micro-nutrient functioning as an antioxidant. When administered at a mega-dose, vitamin C has been
Qian Kong et al.
Experimental and therapeutic medicine, 17(5), 3598-3606 (2019-04-17)
Acute lung injury (ALI) is a critical syndrome that is associated with high morbidity and mortality rates. The activation of the Fas/Fas ligand (FasL) signaling pathway may be an important pathophysiological mechanism during ALI development. Penehyclidine hydrochloride (PHC) has been

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