Recommended Products
![Guanosine 5′-[γ-thio]triphosphate tetralithium salt ≥90% (contains < 10% GDP, HPLC), powder](/deepweb/assets/sigmaaldrich/product/structures/131/514/e3025b6a-cb52-4818-b20f-98efac485c1a/640/e3025b6a-cb52-4818-b20f-98efac485c1a.png)
shipped in
dry ice
storage temp.
−20°C
General description
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families.
The Transcreener® GDP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of GDP and therefore is compatible with any enzyme that produces GDP, including GTPases such as CDC42. The Transcreener® GDP Assay is a simple one step homogenous detection assay, and is extremely flexible with regard to GTP concentration (1 to 1,000 μM GTP). The assay provides excellent signal at low substrate conversion, with a Z′ = 0.7 and =85 polarization shift (mP) at 10% GTP conversion using 10 μM GTP.
The Transcreener® GDP Assay was developed to follow the progress of any enzyme that produces GDP. The Transcreener® GDP Detection Mixture comprises a GDP Alexa633 Tracer bound to a GDP Antibody. The tracer is displaced by GDP, the invariant product generated during the enzyme reaction. The displaced tracer freely rotates leading to a decrease in fluorescence polarization. Therefore, GDP production is proportional to a decrease in polarization. The assay uses a far red tracer to minimize interference from fluorescent compounds and light scattering.
View full Transcreener® product list
The Transcreener® GDP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of GDP and therefore is compatible with any enzyme that produces GDP, including GTPases such as CDC42. The Transcreener® GDP Assay is a simple one step homogenous detection assay, and is extremely flexible with regard to GTP concentration (1 to 1,000 μM GTP). The assay provides excellent signal at low substrate conversion, with a Z′ = 0.7 and =85 polarization shift (mP) at 10% GTP conversion using 10 μM GTP.
The Transcreener® GDP Assay was developed to follow the progress of any enzyme that produces GDP. The Transcreener® GDP Detection Mixture comprises a GDP Alexa633 Tracer bound to a GDP Antibody. The tracer is displaced by GDP, the invariant product generated during the enzyme reaction. The displaced tracer freely rotates leading to a decrease in fluorescence polarization. Therefore, GDP production is proportional to a decrease in polarization. The assay uses a far red tracer to minimize interference from fluorescent compounds and light scattering.
View full Transcreener® product list
Quantity
3009-1K = 1,000 assay, 384-well
3009-10K = 10,000 assay, 384-well
3009-10K = 10,000 assay, 384-well
Physical form
Kit with buffered aqueous solutions
Legal Information
Transcreener is a registered trademark of BellBrook Labs
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Kristel Berg et al.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 33(4-5), 169-185 (2020-07-11)
Iron is an essential nutrient for bacteria, however its propensity to form toxic hydroxyl radicals at high intracellular concentrations, requires its acquisition to be tightly regulated. Ferric uptake regulator (Fur) is a metal-dependent DNA-binding protein that acts as a transcriptional
Laurette Pinkerton et al.
Microorganisms, 7(12) (2019-12-15)
Reducing acute mortality in aquatic crustaceans using natural alternatives to antibiotics has become a necessity, firstly for its positive impact on the aquaculture industry and, secondly, because the extensive use of antibiotics may lead to increased levels of drug resistance
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service