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biological source
rabbit
Quality Level
100
500
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
SP92, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (294R-14)
vial of 0.5 mL concentrate (294R-15)
bottle of 1.0 mL predilute (294R-17)
vial of 1.0 mL concentrate (294R-16)
bottle of 7.0 mL predilute (294R-18)
manufacturer/tradename
Cell Marque™
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
isotype
IgG1
control
B-cell lymphoma, tonsil
shipped in
wet ice
storage temp.
2-8°C
visualization
nuclear
Gene Information
human ... POU2AF1(5450)
Related Categories
General description
Expression of BOB.1/OBF.1 is restricted largely to mature B-cells. Germinal center B-cells normally demonstrate strong staining for BOB.1, as do mantle-zone B-cells, and plasma cells. Analyses of BOB.1/OBF.1 expression in a variety of established B-cell lines representing different stages of B-cell development has suggested a constitutive, B-cell-specific expression pattern. Because they are germinal center derived, L&H cells in nodular lymphocyte predominant Hodgkin lymphoma are consistenty immunoreactive for BOB.1. Conversely, the Hodgkin/Reed- Sternberg cells in classical Hodgkin lymphoma either do not express both (80%) or express only one (20%) of the two proteins. In B-cell lymphomas, the highest expression levels for BOB.1/OBF.1 are reported in follicular center lymphomas, diffuse large B-cell lymphomas and Burkitt lymphomas.B-CLL, MALT-type, and mantle cell lymphomas score negative or display an heterogenous/weaker reactivity.10-11 Studies have suggested that anti-BOB. has a high predictive value in discriminating primary mediastinal B-cell lymphoma from classical Hodgkin disease.Approximately 50% of acute myeloid leukemias express BOB.1.
Linkage
BOB.1 Positive Control Slides, Product No. 294S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Other Notes
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
Legal Information
Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany
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Regulatory Information
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Certificates of Analysis (COA)
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A Greiner et al.
The American journal of pathology, 156(2), 501-507 (2000-02-10)
The BOB.1/OBF.1/OCAB.1 protein is a lymphocyte-specific transcriptional coactivator. It interacts with the Oct1 and Oct2 transcription factors and contributes to the transcriptional activity of octamer motifs. The analysis of established B cell lines had suggested that BOB.1/OBF.1 is constitutively expressed
Ana-Isabel Sáez et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 15(3), 211-220 (2002-03-21)
Oct1 and Oct2 are transcription factors of the POU homeo-domain family that bind to the Ig gene octamer sites, regulating B-cell-specific genes. The function of these transcription factors is dependent on the activity of B-cell-restricted coactivators such as BOB.1/OBF.1. Independent
Susanne Annette Steimle-Grauer et al.
Virchows Archiv : an international journal of pathology, 442(3), 284-293 (2003-03-21)
The World Health Organization (WHO) classification of Hodgkin lymphoma (HL) distinguishes two types: Classical Hodgkin lymphoma (CHL) and nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). Both groups have in common that they mostly derive from B cells with rare classical cases
Stefano A Pileri et al.
The American journal of pathology, 162(1), 243-253 (2003-01-01)
Although primary mediastinal (thymic) large B-cell lymphoma has been primarily studied, its precise phenotype, molecular characteristics, and histogenesis are still a matter of debate. The International Extranodal Lymphoma Study Group collected 137 such cases for extensive pathological review. Histologically, the
Christina B Hertel et al.
Oncogene, 21(32), 4908-4920 (2002-07-16)
In classical Hodgkin lymphoma the malignant Hodgkin/Reed-Sternberg (HRS) cells characteristically constitute only a small minority of the tumour load. Their origin has been debated for decades, but on the basis of rearrangement and somatic hypermutations of their immunoglubulin (Ig) genes
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