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25740

Sigma-Aldrich

Chlorophyll b from spinach

~95% (HPLC)

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About This Item

Empirical Formula (Hill Notation):
C55H70MgN4O6
CAS Number:
519-62-0
Molecular Weight:
907.47
Beilstein:
4122778
EC Number:
208-272-4
MDL number:
MFCD00079053
UNSPSC Code:
12352200
PubChem Substance ID:
57648147

Assay

~95% (HPLC)

form

powder

mp

183-185 °C (lit.)

shipped in

dry ice

storage temp.

−20°C

SMILES string

[H]C(=O)c1c(CC)c2cc3c(C)c4C(=O)[C@H](C(=O)OC)c5c6nc(cc7c(C)c(C=C)c(cc1n2)n7[Mg]n3c45)[C@@H](C)[C@@H]6CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C

InChI

1S/C55H71N4O6.Mg/c1-12-38-35(8)42-27-43-36(9)40(23-24-48(61)65-26-25-34(7)22-16-21-33(6)20-15-19-32(5)18-14-17-31(3)4)52(58-43)50-51(55(63)64-11)54(62)49-37(10)44(59-53(49)50)28-46-39(13-2)41(30-60)47(57-46)29-45(38)56-42;/h12,25,27-33,36,40,51H,1,13-24,26H2,2-11H3,(H-,56,57,58,59,60,62);/q-1;+2/p-1/b34-25+;/t32-,33-,36+,40+,51-;/m1./s1

InChI key

NSMUHPMZFPKNMZ-VBYMZDBQSA-M

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General description

Chlorophyll b is one of the chlorophyll pigments that impart leaves their green color. Chlorophyll is a Greek name with chlorós meaning green and phyllon meaning leaf. It is present in peripheral antenna complexes along with chlorophyll a. The amount of chlorophyll b in spinach is found to be lesser than chlorophyll a.

Biochem/physiol Actions

Chlorophyll b undergoes breakdown to form pheophytin b by the enzyme chlorophyllase. This causes a change in its color from green to olive-brown. It is involved in the harvesting of light energy during the process of photosynthesis and its subsequent conversion to chemical energy. Along with chlorophyll a, it plays a key role in the ability of a plant to adapt to different light intensities.
Chlorophyll b extends the absorption spectrum of light-harvesting complexes LHC-I and LHC-II above 700 nm, a region of the visible spectrum that is not absorbed when only chlorophyll a is present in the LHCs.

Other Notes

Dielectric properties in nematic liquid crystals; Polarization spectra; Characterization; Note: anhydrous chlorophylls are very easily enolized, epimerized and allomerized in polar organic solvents (dry).

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLBP6239V
SLBR9799V
SLBQ9734V
SLBN5606V
SLBF7339V

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R. Avarmaa et al.
Opt. Spectrosk., 56, 53-53 (1984)
R.M. Leblanc et al.
Photobiochem. Photobiophys., 7, 41-41 (1984)
I. Sahlberg et al.
Journal of Chromatography A, 291, 331-331 (1984)
P.H. Hynninen et al.
Synthesis, 705-705 (1983)
N I Adamchuk-Chalaia
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993), 74(6), 3-8 (2013-01-09)
The morphological and functional changes of photosynthetic apparatus of spring wheat at inoculation by active strains of Aospirillium genus diazotrophs were studied. Inoculation resulted in an increase of mesophyll cell size of 3 spring wheat leaves and chloroplasts in them.
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