22178
Cellulase from Aspergillus niger
powder, off-white, ~0.8 U/mg
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biological source
Aspergillus niger
Quality Level
form
powder
specific activity
~0.8 U/mg
color
off-white
storage temp.
2-8°C
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General description
Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.
Application
Cellulase from Aspergillus niger has been used:
- in the preparation of the standardized solution to determine absolute cellulase levels
- in phosphate-citric acid buffer for activity assay
- in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed
Biochem/physiol Actions
Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.
Unit Definition
1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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M.A. Sawsan et al.
Zentralblatt Fur Mikrobiologie, 143, 55-55 (1988)
María Dolores Ayala et al.
Animals : an open access journal from MDPI, 10(12) (2020-12-05)
A 90-d feeding trial was conducted in which five groups of gilthead seabream (11.96 g initial body weight) were fed with a microalgae-free diet (control group, C) or four diets containing the microalgae Nannochloropsis gaditana at two inclusion levels (2.5%
The use of surrogate standards as a QA/QC tool for routine analysis of microplastics in sewage sludge.
Philipp, et al.
The Science of the Total Environment, 835, 155485-155485 (2022)
Helena Fernandes et al.
Bioresource technology, 281, 277-285 (2019-03-03)
The macroalgae aquaculture industry has grown up in the last years, and new applications for macroalgae should be considered. In this work, sequential biological treatments as solid-state fermentation (SSF) by Aspergillus ibericus and enzymatic hydrolysis (EH) were applied to washed
P. Toussaint et al.
Entropie, 23 (136), 8-8 (1987)
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