Skip to Content
Merck
CN
All Photos(1)

Documents

19123

Sigma-Aldrich

Nile Red

suitable for fluorescence, BioReagent, ≥97.0% (HPLC)

Synonym(s):

Nile Blue A Oxazone

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C20H18N2O2
CAS Number:
7385-67-3
Molecular Weight:
318.37
Beilstein:
279110
EC Number:
230-966-0
MDL number:
MFCD00011639
UNSPSC Code:
12161900
PubChem Substance ID:
57647780
NACRES:
NA.25

product line

BioReagent

Quality Level

100

Assay

≥97.0% (HPLC)

form

solid

mp

203-205 °C (lit.)
205-215 °C

λmax

553 nm

fluorescence

λex 530 nm; λem 635 nm in methanol

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChI key

VOFUROIFQGPCGE-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Nile Red is an uncharged hydrophobic molecule and a benzophenoxazone dye. It functions as a fluorescent probe for intracellular lipids and hydrophobic domains of proteins. This dye is fluorescent in all organic solvents. Its fluorescence colors range from golden yellow to deep red.

Application

Nile Red has been used for the staining of intracellular lipid droplets and neutral lipids.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Nile Red, Product 19123, soluble in?

    Nile Red is soluble in methanol (1 mg/mL), ethanol (1mg/mL), water (0.2 mg/mL), and 2-methoxyethanol (1mg/mL).

  4. How should Nile Red, Product 19123, be stored?

    We recommend storing this product at 2-8 °C, which is in accordance with our MSDS.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces
Sackett D L and Wolff J
Analytical Biochemistry, 167(2), 228-234 (1987)
Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
Feaver RE, et al.
JCI insight, 1(20) (2016)
Resveratrol suppresses T0901317-induced hepatic fat accumulation in mice
Gao M and Liu D
The AAPS Journal, 15, 744-752 (2013)
Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes
Kim K, et al.
Scientific Reports, 6, 36815-36815 (2016)
Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation
Shen XF, et al.
Water Research, 81(3), 294-300 (2015)
View All Related Papers

Articles

Stimuli-Responsive Materials as Intelligent Drug Delivery Systems

By altering the physicochemical properties, smart or intelligent drug delivery systems can be designed to deliver therapeutic molecules on-demand. Learn more about the application of stimuli-responsive materials in drug delivery.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service