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18373

Sigma-Aldrich

Atto 647N NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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1 MG
CN¥637.75
5 MG
CN¥2,192.72

CN¥637.75

Available to ship onApril 16, 2025Details

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1 MG
CN¥637.75
5 MG
CN¥2,192.72

About This Item

MDL number:
MFCD07370582
UNSPSC Code:
12352108
NACRES:
NA.32

CN¥637.75

Available to ship onApril 16, 2025Details

Request a Bulk Order

product line

BioReagent

Assay

≥90% (HPLC)
≥90% (degree of coupling)

form

solid

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 641-647 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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EHU067331EHU092821EHU114811
MISSION® esiRNA targeting human TRIM29

EHU087831

MISSION® esiRNA

MISSION® esiRNA targeting human TRIM28

EHU067331

MISSION® esiRNA

MISSION® esiRNA targeting human TRIM69

EHU092821

MISSION® esiRNA

MISSION® esiRNA targeting human TRIM26

EHU114811

MISSION® esiRNA

esiRNA cDNA target sequence

TCATGGATGCTCTGGATGAGAGAGCCAAGGTGCTGCATGAGGACAAGCAGACCCGGGAGCAGCTGCATAGCATCAGCGACTCTGTGTTGTTTCTGCAGGAATTTGGTGCATTGATGAGCAATTACTCTCTCCCCCCACCCCTGCCCACCTATCATGTCCTGCTGGAGGGGGAGGGCCTGGGACAGTCACTAGGCAACTTCAAGGACGACCTGCTCAATGTATGCATGCGCCACGTTGAGAAGATGTGCAAGGCGGACCTGAGCCGTAACTTCATTGAGAGGAACCACATGGAGAACGGTGGTGACCATCGCTATGTGAACAACTACACGAACAGCTTCGGGGGTGAGTGGAGTGCACCGGACACCATGAAGAGATACTCCATGTACCTGACACCCAAAGGTGGGGTCCGGACATCATACCAGCCCTCGTC

esiRNA cDNA target sequence

CACAAGGACCACCAGTACCAGTTCTTAGAGGATGCAGTGAGGAACCAGCGCAAGCTCCTGGCCTCACTGGTGAAGCGCCTTGGGGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCAATCCGCCAGGTGTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGCAGATCATGAAGGAGCTGAATAAGCGGGGCCGTGTGCTGGTCAATGATGCCCAGAAGGTGACTGAGGGGCAGCAGGAGCGCCTGGAGCGGCAGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCACATTCTGCGCTTTGCCTCTTGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTCTAAGAAGTTGATCTACTTCCAGCTGCACCG

esiRNA cDNA target sequence

CGTCTGGCATGGTGACATTAAGAAGATAATGCCTGATGATCCTGAGAGGTTTGACTCAAGTGTGGCTGTACTGGGCTCAAGAGGCTTCACCTCTGGAAAGTGGTACTGGGAAGTAGAAGTAGCAAAGAAGACAAAATGGACAGTTGGAGTTGTCAGAGAATCCATCATTCGGAAGGGCAGCTGTCCTCTAACTCCTGAGCAAGGATTCTGGCTTTTAAGACTAAGGAACCAAACTGATCTAAAGGCTCTGGATTTGCCTTCTTTCAGTCTGACACTGACTAACAACCTCGACAAGGTGGGCATATACCTGGATTATGAAGGAGGACAGTTGTCCTTCTACAATGCTAAAACCATGACTCACATTTACACCTTCAGTAACACTTTCATGGAGAAACTTTATCCCTACTTCTGCCCCTGCCTTAAT

esiRNA cDNA target sequence

CAGCGCATATCATCTCATGGGGTCCAGAGTGGAGTCCAGGCTTCTCTGAAACCAGGGCTGAGCATATTTCCATAGCCAAGGAGGTGGGACTCCCTGGAGCTATCTGTGGTCTTAGGGAAGGATCCAGACATACACGGCTTTGGGGTACAAGCTGTGATCACTGATCAATAAATTATCTCTAGATTGGTCCTTGTGAGGGGAGTTTTAAGAATCCAGAACATCTTGCTCTTGATCAGCACATCCAAAAACACCAAGACAAACATCCAGTGGAGCAGAACCTCTCCTGCCTCGGGAGTTCTGACCGGGTTCCCCAGCAGGGTGTTAAGCCTCTGTCTCTGGCCCTACCAGCATCCAGGTTCCACTTTCCTAGGAGAGAGTGGAGATGGGAAGACAGGGAAAGGAAGG

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

Gene Information

human ... TRIM29(23650), TRIM29(23650)

Gene Information

human ... TRIM28(10155), TRIM28(10155)

Gene Information

human ... TRIM69(140691), TRIM69(140691)

Gene Information

human ... TRIM26(7726), TRIM26(7726)

NCBI accession no.

NM_012101

NCBI accession no.

NM_005762

NCBI accession no.

NM_080745

NCBI accession no.

NM_003449

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

Atto 647N is a superior red-emitting fluorescence dye with a strong absorption, excellent fluorescence quantum yield (65%), high photostability, excellent ozone resistance, good solubility, and very little triplet formation.

Application

Atto 647N belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, high photostability, excellent ozone resistance, good solubility, and very little triplet formation. Atto 647N is a cationic dye. The active ester of Atto 647N fluorescence dye reacts with amino groups under mild conditions. After coupling to a substrate the dye carries a net electrical charge of +1. In common with most Atto-labels, absorption and fluorescence are independent of pH in the range of 2 to 11, used in typical applications. As supplied Atto 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    BCCN0571
    BCCM3393
    BCCM9221
    BCCM9220
    BCCM6545

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    Shangguo Hou et al.
    Nature communications, 11(1), 3607-3607 (2020-07-19)
    To date, single molecule studies have been reliant on tethering or confinement to achieve long duration and high temporal resolution measurements. Here, we present a 3D single-molecule active real-time tracking method (3D-SMART) which is capable of locking on to single
    Jialei Tang et al.
    Scientific reports, 7(1), 10945-10945 (2017-09-10)
    We report a simple single-molecule fluorescence imaging method that increases the temporal resolution of any type of array detector by >5-fold with full field-of-view. We spread single-molecule spots to adjacent pixels by rotating a mirror in the detection path during
    Jennifer Z Yao et al.
    Journal of the American Chemical Society, 134(8), 3720-3728 (2012-01-14)
    Methods for targeting of small molecules to cellular proteins can allow imaging with fluorophores that are smaller, brighter, and more photostable than fluorescent proteins. Previously, we reported targeting of the blue fluorophore coumarin to cellular proteins fused to a 13-amino
    Ioannis Sgouralis et al.
    The journal of physical chemistry. B, 123(3), 675-688 (2018-12-21)
    We develop a Bayesian nonparametric framework to analyze single molecule FRET (smFRET) data. This framework, a variation on infinite hidden Markov models, goes beyond traditional hidden Markov analysis, which already treats photon shot noise, in three critical ways: (1) it
    Feng Ye et al.
    The Journal of endocrinology, 206(1), 27-36 (2010-04-16)
    Adipose tissue is a highly active endocrine organ secreting a variety of signaling molecules called adipokines. Leptin and resistin are two adipokines critically involved in metabolic homeostasis. Nevertheless, the secretory pathways of these adipokines and their interplays are poorly elucidated.
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