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171R-1

Sigma-Aldrich

CD71 (EP232) Rabbit Monoclonal Primary Antibody

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Synonym(s):
TFR1, TRF, TRFR, Transferrin Receptor 1, p90, transferrin receptor (p90, CD71)
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

EP232, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (171R-14)
vial of 0.1 mL concentrate Research Use Only (171R-14-RUO)
vial of 0.5 mL concentrate (171R-15)
vial of 1.0 mL concentrate (171R-16)
vial of 1.0 mL concentrate Research Use Only (171R-16-RUO)
vial of 1.0 mL pre-dilute Research Use Only (171R-17-RUO)
vial of 1.0 mL predilute ready-to-use (171R-17)
vial of 7.0 mL pre-dilute ready-to-use Research Use Only (171R-18-RUO)
vial of 7.0 mL predilute ready-to-use (171R-18)

manufacturer/tradename

Cell Marque™

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200 (concentrated)

isotype

IgG

control

bone marrow

shipped in

wet ice

storage temp.

2-8°C

visualization

cytoplasmic, membranous

Related Categories

General description

Transferrin receptor 1 (CD71) is expressed on placental syncytiotrophoblasts, myocytes, basal keratinocytes, hepatocytes, endocrine pancreas, spermatocytes, and erythroid precursors. The level of transferrin receptor expression is highest in early erythroid precursors through the intermediate normoblast phase, after which expression decreases through the reticulocyte phase. The maturation of erythrocytes results in loss of transferrin receptor expression. AntiCD71 is useful in identifying erythroid precursors. The high level of transferrin receptor within erythroid precursors makes anti-CD71 an excellent marker for evaluation of erythroid precursors within bone marrow biopsy specimens and shows the following features: 1) distinct membranous and cytoplasmic staining pattern, which is easily recognized in bone marrow biopsy; 2) restriction to erythroid lineage within bone marrow biopsy specimens; 3) CD71 expression decreases with the maturation of erythrocytes, with the highest level seen in early forms and the lowest level in late normoblast stage, and most importantly; 4) mature erythrocytes do not express CD71.

Quality

United States - IVD
Canada - IVD
European Union - IVD
Japan - RUO

Linkage

CD71 Positive Control Slides, Product No. 171S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Predilute: diluted in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Concentrate: diluted in Phosphate Buffered Saline, pH 7.2, with 1% BSA and <0.1% Sodium Azide

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers.
Nakahata T and Okumura N
Leukemia & Lymphoma, 13(5-6), 401-409 (1994)
Changes in cell surface antigen expression during hemopoietic differentiation.
Sieff C, Bicknell D, Caine G, et al.
Blood, 60(3), 703-713 (1982)
J Lesley et al.
Cellular immunology, 83(1), 14-25 (1984-01-01)
We have used a monoclonal antibody against the murine transferrin receptor to study the expression of the transferrin receptor on the hematopoietic progenitor cells (BFU-E, CFU-E, and CFU-C) present in mouse bone marrow. Elutriation and cell-sorting data are consistent with
C Sieff et al.
Blood, 60(3), 703-713 (1982-09-01)
Human bone marrow cells were separated on a fluorescence activated cell sorter (FACS) according to their binding of a series of monoclonal antibodies; the positive and negative fractions were cloned for erythroid burst and colony-forming units (BFU-E and CFU-E) and
Expression of transferrin receptor on murine hematopoietic progenitors.
Lesley J, Hyman R, Schulte R, et al.
Cellular Immunology, 83(1), 14-25 (1984)
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