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Merck
CN

16805

Atto 425 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

Atto 425-N-hydroxysuccinimide ester

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About This Item

Empirical Formula (Hill Notation):
C26H30N2O8
CAS Number:
892156-28-4
Molecular Weight:
498.53
NACRES:
NA.32
PubChem Substance ID:
57647590
UNSPSC Code:
12352108
MDL number:
MFCD04974819

Key Documents

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Product Name

Atto 425 NHS ester, BioReagent, suitable for fluorescence, ≥90% (HPLC)

Quality Level

100

InChI

1S/C26H30N2O8/c1-5-34-24(32)18-12-16-11-17-15(2)14-26(3,4)27(19(17)13-20(16)35-25(18)33)10-6-7-23(31)36-28-21(29)8-9-22(28)30/h11-13,15H,5-10,14H2,1-4H3

SMILES string

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)ON4C(=O)CCC4=O)c3cc2OC1=O

InChI key

PUEQEMDTFPYCDY-UHFFFAOYSA-N

product line

BioReagent

assay

≥90% (HPLC)
≥90% (degree of coupling)

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 422-428 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Related Categories

Application

Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation .
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

General description

Atto 425 is a member of the commercially available coumarin derivatives.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Other Notes

Study of stability during one- and two-photon excitation in fluorescence correlation spectroscopy, FCS; employed in triple-color coincidence analysis.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN5811
BCCN4631
BCCN2267
BCCM6578
BCCM6546

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Handbook of Biophotonics: Vol. 2: Photonics for Health Care (2011)
P.S. Dittrich, P. Schwille
Applied Physics B: Lasers and Optics, 73, 829-829 (2001)
Katrin G Heinze et al.
Biophysical journal, 86(1 Pt 1), 506-516 (2003-12-26)
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence
Triple FRET: a tool for studying long-range molecular interactions.
Elke Haustein et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 745-748 (2003-08-07)
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
Benzothiazole dye thioflavin T (ThT) is a sensitive probe for amyloid fibril detection. The ThT probing is based on its unique ability to form highly fluorescent complexes with amyloid and amyloid-like fibrils. In this work we propose an approach of
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