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BIS-TRIS propane

EMPROVE® EVOLVE

Pharma Manufacturing

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Synonym(s):
BIS-TRIS propane, 1,3-Bis[tris(hydroxymethyl)methylamino]propane
Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
64431-96-5
Molecular Weight:
282.33
Beilstein:
1786109
MDL number:
MFCD00004689
UNSPSC Code:
12161705

biological source

synthetic

Quality Level

400

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

pH

6.3-9.5

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

pKa 

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)
164-165 °C (lit.)

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

storage temp.

2-30°C

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

Related Categories

Application

Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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