11610
Azo-Casein
protease substrate
Synonym(s):
Azocasein
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About This Item
biological source
bovine
description
protease substrate
technique(s)
electrophoresis: suitable
solubility
water: 5 mg/mL
ε (extinction coefficient)
32-38 at 440 nm in 0.1 M NaOH at 1% (1 cm)
storage temp.
2-8°C
General description
Chromogenic substrate for proteolytic activity; Digestion results in the formation of colored components, which are soluble in trichloroacetic acid and determined (after neutralization) in the range 366 to 450 nm.
Application
Suitable as substrate for proteolytic enzymes and useful for post-electrophoretic detection of proteases.
Biochem/physiol Actions
Can be used to induce secondary amyloidosis in mice, in studies of amyloid protein processing.
Other Notes
Sales restrictions may apply
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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D Vázquez Peyronel et al.
Electrophoresis, 16(10), 1894-1897 (1995-10-01)
Azocasein, diffused into gels after polyacrylamide gel electrophoresis, provides a useful substrate for simple and rapid protease detection. After electrophoresis, the gels are incubated in an azocasein solution, immersed in trichloroacetic acid and then treated with NaOH. Proteolytic zones appear
Pineapple cysteine endopeptidases.
A D Rowan et al.
Methods in enzymology, 244, 555-568 (1994-01-01)
Fanqi Wang et al.
Immunology, 159(1), 109-120 (2019-10-14)
Serpins are evolutionarily conserved serine protease inhibitors that are widely distributed in animals, plants and microbes. In this study, we reported the cloning and functional characterizations of two novel serpin genes, HlSerpin-a and HlSerpin-b, from the hard tick Haemaphysalis longicornis
Shih-Hua Lo et al.
Animal bioscience, 35(10), 1592-1605 (2022-04-26)
The objective of this study was to select an effective in vitro digestion-fermentation model to estimate the effect of decreasing dietary crude protein (CP) on odor emission during pig production and to suggest potential prediction markers through in vitro and
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