11371
Avidin-Peroxidase Conjugate from hen egg white/horseradish
lyophilized, powder, almost white, avidin ~5 U/mg
Synonym(s):
Avidin-POD
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Form:
powder
form
powder
quality
lyophilized
avidin activity
~5 U/mg
impurities
≥80 U/mg peroxidase (further activity)
color
almost white
storage temp.
−20°C
Preparation Note
Conjugate Description: ~1.5 moles peroxidase/mol avidin; MBS-linkage (Cat. No.No. 63173)
Other Notes
1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5 further activity: peroxidase ≥80 U/mg [1U corresponds 1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C]
Staining immunoblots with biotinylated primary antibodies
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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H.J. Willison et al.
IRCS Med. Sci., 14, 201-201 (1986)
E Lacey et al.
Analytical biochemistry, 163(1), 151-158 (1987-05-15)
A sensitive method for the nonisotopic in vitro labeling of proteins under physiological conditions using photobiotin, a compound originally developed for labeling nucleic acids (Forster et al. (1985) Nucleic Acids Res. 13, 745), has been developed. Using sheep brain tubulin
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