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11012008

SK-GT-2 cell line

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UNSPSC Code:
41106514

biological source

human esophagus

Quality Level

100

growth mode

Adherent

karyotype

Modal number 58 chromosomal aberration at 11p13-15.

morphology

Epithelial

products

Expression of actin imtermediate filaments and growth factors TGF?1, TGF?2, TGF?3, TGF? and platelet-derived growth factor A (PDGFA).

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Adenocarcinoma of the Gastric fundus, poorly differentiated

Cell Line Description

SK-GT-2 was established from a primary adenocarcinoma of gastric fundus from a 72-year-old Hispanic male previously treated for squamous cell carcinoma with radiation therapy and radical neck dissection. Pathology of recurrence (1 year) demonstrated ulcerating poorly differentiated adenocarcinoma of the stomach invading spleen and epigastic fat (T4) with metastases to perisplenic and perigastric nodes (N1). SK-GT-2 was found to be tumorigenic in athymic nu/nu mice. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 11,12
D5S818: 10
D7S820: 9,10
THO1: 8,9
TPOX: 9,12
vWA: 15,18

Culture Medium

RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 44hrs.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Jurjen J Boonstra et al.
Journal of the National Cancer Institute, 102(4), 271-274 (2010-01-16)
For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the

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