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10831

Sigma-Aldrich

Histopaque®-1083

sterile-filtered, density: 1.083 g/mL

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UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

400

form

liquid

shelf life

Expiry date on the label.

impurities

endotoxin, tested

density

1.083 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

Related Categories

General description

Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Application

Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Features and Benefits

Histopaque®-1083 offers numerous advantages, such as:

  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Principle

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Are there limitations when attempting to use Product 10831, Histopaque®-1083 with the various Accuspin products?

    Yes.  There are some limitations.  Small amounts of blood cannot be processed in the Accuspin tubes.  At least 3 mls of whole peripheral blood is expected.  Without sufficient red cell numbers, the mononuclear band fails to adequately clear the barrier or frit found within the Accuspin tubes.

  6. I find mononuclear cells separate well from human blood over Product 10831, Histopaque®-1083, but the cell recovery from rats and mice is lower.  Why?

    The most common cause for separation problems with Histopaque® 1083 is the blood source.  Blood from rats and mice is harder to obtain than blood from a human.  Most users experiencing problems are collecting blood with a syringe.  It is relatively uncommon for users to call in about poor separation when using vacuum tubes.  Humanely sacrifice the animal if possible and immediately collect the blood from the aorta or vena cava. Collecting blood from a severed head is never acceptable.  Blood obtained from bleeding the tail is also generally unacceptable.  Tissue fluids and other extraneous debris will often result in an activation of the coagulation cascase.  Blood drawn into a vacuum tube is the best source of blood.  See the product insert for Histopaque® 1083 for the amount of anticoagulant to add to blood collected with a syringe.

  7. Can Product 10831, Histopaque®-1083, be used with human blood?

    Cells can be recovered from human blood using Histopaque® 1083.  However Sigma sells Histopaque® 1077 specifically for use with human blood.  Histopaque® 1077 is intended for In Vitro Diagnostic Use.  Histopaque® 1083 is intended for research use only with animal blood.

  8. Is there any data for expected percent recovery with rat or mouse blood when using Product 10831, Histopaque®-1083?

    No.  Histopaque® 1083 is a research use product and no studies have been performed to determine percent recovery.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Ulrich Flögel et al.
Circulation, 118(2), 140-148 (2008-06-25)
In this study, we developed and validated a new approach for in vivo visualization of inflammatory processes by magnetic resonance imaging using biochemically inert nanoemulsions of perfluorocarbons (PFCs). Local inflammation was provoked in 2 separate murine models of acute cardiac
Gangduo Wang et al.
Toxicology and applied pharmacology, 237(2), 188-195 (2009-04-01)
Even though reactive oxygen and nitrogen species (RONS) are implicated as mediators of autoimmune diseases (ADs), little is known about contribution of protein oxidation (carbonylation and nitration) in the pathogenesis of such diseases. The focus of this study was, therefore
Bella S Guerrouahen et al.
Pediatric blood & cancer, 53(6), 1132-1135 (2009-06-02)
Tyrosine kinase inhibitors, such as imatinib, have dramatically improved the outcomes for patients with selected cancers. For imatinib, western blotting of phospho-CrkL was an insensitive, indirect, and descriptive method to determine drug efficacy. Greater use of targeted therapies should involve
Karin Knieke et al.
PloS one, 4(5), e5702-e5702 (2009-05-30)
Migration of antigen-experienced T cells to secondary lymphoid organs and the site of antigenic-challenge is a mandatory prerequisite for the precise functioning of adaptive immune responses. The surface molecule CD152 (CTLA-4) is mostly considered as a negative regulator of T
Timothy J Chapman et al.
PloS one, 6(5), e20493-e20493 (2011-06-08)
After infection, extralymphoid tissues are enriched with effector and memory T cells of a highly activated phenotype. The capacity for rapid effector cytokine response from extralymphoid tissue-memory T cells suggests these cells may perform a 'sentinel' function in the tissue.
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