Skip to Content
Merck
CN

Key Documents

View All Documentation

10831

Histopaque®-1083

sterile-filtered, density: 1.083

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

sterility

sterile-filtered

Quality Segment

400

form

liquid

shelf life

Expiry date on the label.

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

density

1.083

application(s)

hematology
histology

storage temp.

2-8°C

General description

Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a relative density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Application

Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Biochem/physiol Actions

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Features and Benefits

Histopaque®-1083 offers numerous advantages, such as:

  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library