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Human Dermal Fibroblasts: HDF, neonatal

Synonym(s):

HDF cells

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human neonatal foreskin or adult skin (normal)

Quality Level

100

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

Fibroblast

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)

Cell Line Origin

Skin

Application

injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response

Components

Fibroblast Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine

Please refer to the HDF Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

高风险级别生物产品--人源产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jitka Viktorova et al.
PloS one, 14(5), e0212718-e0212718 (2019-05-11)
Although many genetic manipulations of crops providing biofortified or safer food have been done, the acceptance of biotechnology crops still remains limited. We report on a transgenic barley expressing the multi-functional protein osmotin that improves plant defense under stress conditions.
Fabio Sabino et al.
Cell death & disease, 11(8), 674-674 (2020-08-23)
Proteases modulate critical processes in cutaneous tissue repair to orchestrate inflammation, cell proliferation and tissue remodeling. However, the functional consequences and implications in healing impairments of most cleavage events are not understood. Using iTRAQ-based Terminal Amine Isotopic Labeling of Substrates

Protocols

Human Dermal Fibroblasts (HDF) Culture Protocol

This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF

Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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