104-05A
Human Epidermal Melanocytes: HEM, adult
Synonym(s):
HEM cells
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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
biological source
human skin (normal abdominal)
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 46
morphology
Epidermal
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metabolic disorders
shipped in
dry ice
storage temp.
−196°C
Quality Level
Application
melanin production, melanocyte proliferation, differentiation
Biochem/physiol Actions
Skin
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description
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Epidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia.
Epidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia.
Other Notes
Basal Medium containing 30% FBS & 10% DMSO
Preparation Note
- 2nd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Please refer to the HEM Culture Protocol.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Protocols
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
将细胞放入冻存管后须立即储存在液氮储罐中。
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