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10115

Sigma-Aldrich

Amyloglucosidase from Aspergillus niger

greener alternative

lyophilized, powder, ~70 U/mg

Synonym(s):

1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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About This Item

CAS Number:
9032-08-0
Enzyme Commission number:
3.2.1.3 (BRENDA, IUBMB)
EC Number:
232-877-2
MDL number:
MFCD00081350
UNSPSC Code:
12352204
NACRES:
NA.54

form

powder

Quality Level

200

quality

lyophilized

specific activity

~70 U/mg

mol wt

Mr ~97,000

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

parameter

temperature at 50 °C (optimum temperature)

greener alternative category

, Enabling

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Amyloglucosidase is a disaccharide enzyme belonging to the α-glucosidase type. It is produced by several species of Aspergillus genus.

Application

Amyloglucosidase from Aspergillus niger has been used in glycogen assay and in acetate buffer for starch hydrolysis. It can also be used for glycogen determination in whole yeast cells and synthesis of hetero-oligosaccharides by glucoamylase in reverse.

Unit Definition

One unit corresponds to the amount of enzyme which liberates 1 μmole of glucose per minute at pH 4.8 and 60 °C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of Product 10115,  Amyloglucosidase from Aspergillus niger?

    Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).

  6. Does Product 10115,  Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?

    No extending additives such as glucose or sucrose are added. Only sodium acetate buffer salts are added. It is unknown if the starting material contained any additives.

  7. How can a solution of Product 10115,  Amyloglucosidase from Aspergillus niger, be prepared?

    A 1 mg/mL solution may be prepared in water, or in 10 mM sodium acetate buffer at approximately pH 4.8.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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