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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
fish fin
Growth mode:
Adherent
Morphology:
Fibroblast
Receptors:
Not specified
Technique(s):
cell culture | mammalian: suitable
biological source
fish fin
packaging
tube of 5 μg 10072801-DNA-5UG, pkg of vial of cells 10072801-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
Quality Level
Biochem/physiol Actions
Koi carp fin cell line, koi herpesvirus isolation
Not specified
The KF1 cell line was developed from Koi carp (Cyprinus carpio koi) fin.
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Preparation Note
EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). If a CO2 incubator is not available, 10 mM HEPES can be included.
Split sub-confluent cultures (70-80%) 1:3 to 1:4, i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 25 °C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
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