10012801
1.1B4 human
human pancreas (islets), Epithelial
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About This Item
UNSPSC Code:
12352207
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Product Name
1.1B4 human,
biological source
human pancreas (islets)
Quality Level
form
liquid
growth mode
Adherent
karyotype
Modal chromosome number 67-71
morphology
Epithelial
products
1.1B4 cells have been shown to express insulin, glucokinase and IAPP by immunocytochemistry. These cells express the GLUT1 glucose transporter.
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
A human pancreatic Beta cell: PANC-1 hybrid cell line. Insulin-secreting cell line.
Cell Line Description
The hybrid cell line1.1B4 was formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line (ECACC catalogue number 87092802).
According to the literature (Vasu et al 2013) (Green et al 2015) 1.1B4 secretes a low level of insulin as detected by radioimmunoassay. 1.1B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology.
The STR profile of 1.1B4 is indistinguishable from the parental PANC-1 Cell Line indicating that the DNA from the pancreatic beta cell fusion partner is below the limit of detection of the STR profiling assay. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
According to the literature (Vasu et al 2013) (Green et al 2015) 1.1B4 secretes a low level of insulin as detected by radioimmunoassay. 1.1B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology.
The STR profile of 1.1B4 is indistinguishable from the parental PANC-1 Cell Line indicating that the DNA from the pancreatic beta cell fusion partner is below the limit of detection of the STR profiling assay. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Culture Medium
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6, i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approximately 20 hours. At confluence 105 cells/cm2 can be expected.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Kate L White et al.
Science advances, 6(50) (2020-12-11)
Characterizing relationships between cell structures and functions requires mesoscale mapping of intact cells showing subcellular rearrangements following stimulation; however, current approaches are limited in this regard. Here, we report a unique application of soft x-ray tomography to generate three-dimensional reconstructions
Patchara Rattanaporn et al.
PeerJ, 8, e9298-e9298 (2020-06-27)
Diabetes mellitus (DM) is a common complication found in β-thalassemia patients. The mechanism of DM in β-thalassemia patients is still unclear, but it could be from an iron overload and increase of some cytokines, such as interleukin1-β (IL-1β) and tumor
Akiyo Yamauchi et al.
Journal of diabetes research, 2015, 173058-173058 (2015-03-15)
Reg (Regenerating gene) gene was originally isolated from rat regenerating islets and its encoding protein was revealed as an autocrine/paracrine growth factor for β cells. Rat Reg gene is activated in inflammatory conditions for β cell regeneration. In human, although
Srividya Vasu et al.
Islets, 5(4), 170-177 (2013-08-30)
The novel human-derived pancreatic β-cell line, 1.1B4 exhibits insulin secretion and β-cell enriched gene expression. Recent investigations of the cellular responses of this novel cell line to lipotoxicity and cytokine toxicity revealed similarities to primary human β cells. The current
Beno W Oppenheimer et al.
PloS one, 11(4), e0152769-e0152769 (2016-04-02)
Obesity is characterized by increased systemic and pulmonary blood volumes (pulmonary vascular congestion). Concomitant abnormal alveolar membrane diffusion suggests subclinical interstitial edema. In this setting, functional abnormalities should encompass the entire distal lung including the airways. We hypothesize that in
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