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09111802

MS-1 cell line

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Synonym(s):
MS1 Cells
UNSPSC Code:
12352200

biological source

human skin

Quality Level

100

form

liquid

growth mode

Suspension

karyotype

Not determined

morphology

Loosely packed floating aggregates with irregular outline and no central necrosis

products

Cell line is CM2B4/MCV T, AE1/AE2, CK20, EMA, CAM5.2, chromogranin & NSE. Cell line is negative for for CK7, synaptophysin, LCA TTF-1, vimentin and CD99,

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human neuroendocrine skin carcinoma

Cell Line Description

MS-1 cells contain a Merkel cell polyomavirus integrated into the genome. See also MKL-1 (Sigma Catalogue number 09111801).

Application

Pathobiology of neuroendocrine skin carcinomas (NSCs) and function of cutaneous Merkel cells. The role of polyomavirus in the development of Merkel cell carcinomas (submitted for publication)

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11,14
D16S539: 9
D5S818: 11
D7S820: 8,9
THO1: 7,9.3
TPOX: 8
vWA: 17

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Seed at 3 x 105 cells/ml in 5% CO2; 37°C Saturation density is approximately 106 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Dr Patrick Moore, University of Pittsburgh Cancer Institute, 5117 Centre Avenue Suite 1.8 Pittsburgh PA 15213

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Huichen Feng et al.
Science (New York, N.Y.), 319(5866), 1096-1100 (2008-01-19)
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction and detected a fusion transcript between
Roland Houben et al.
Journal of virology, 84(14), 7064-7072 (2010-05-07)
Merkel cell carcinoma (MCC) is the most aggressive skin cancer. Recently, it was demonstrated that human Merkel cell polyomavirus (MCV) is clonally integrated in approximately 80% of MCC tumors. However, direct evidence for whether oncogenic viral proteins are needed for

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