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09014

Sigma-Aldrich

DAR-1

BioReagent, suitable for fluorescence, ≥97.0% (HPCE)

Synonym(s):

4,5-Diamino-N,N,N′,N′-tetraethylrhodamine, 4,5-Diamino-rhodamine B

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About This Item

Empirical Formula (Hill Notation):
C28H32N4O3
CAS Number:
Molecular Weight:
472.58
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Quality Level

Assay

≥97.0% (HPCE)

form

powder

fluorescence

λex 566 nm; λem 586 nm in 0.1 M phosphate pH 7.4, NO

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CCN(CC)c1ccc2c(Oc3cc(ccc3C24OC(=O)c5c(N)c(N)ccc45)N(CC)CC)c1

InChI

1S/C28H32N4O3/c1-5-31(6-2)17-9-11-19-23(15-17)34-24-16-18(32(7-3)8-4)10-12-20(24)28(19)21-13-14-22(29)26(30)25(21)27(33)35-28/h9-16H,5-8,29-30H2,1-4H3

InChI key

LFXDWSUQAVSSJH-UHFFFAOYSA-N

Application

suitable as fluorescent NO-sensor

Other Notes

Sensitive fluorescent indicator for nitric oxide, NO, based on rhodamine chromophore; LOD in the region of 10 nM; it is more photostable and emits at longer wave-length than the corresponding derivative of fluorescein (DAF, Cat. No. 41524)

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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H. Kojima et al.
Tetrahedron Letters, 41, 69-69 (2000)

Articles

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

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