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08599

Sigma-Aldrich

N-Biotinyl-ethylenediamine trifluoroacetate salt

≥96.5% (HPLC)

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Synonym(s):
N-(2-Aminoethyl)biotinamide trifluoroacetate salt
Empirical Formula (Hill Notation):
C12H22N4O2S · C2HF3O2
CAS Number:
1217450-40-2
Molecular Weight:
400.42
MDL number:
MFCD11045268
UNSPSC Code:
12352116
PubChem Substance ID:
329748617
NACRES:
NA.32

Quality Level

100

Assay

≥96.5% (HPLC)

solubility

DMSO: soluble
methanol: soluble

storage temp.

2-8°C

SMILES string

OC(=O)C(F)(F)F.NCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12

InChI

1S/C12H22N4O2S.C2HF3O2/c13-5-6-14-10(17)4-2-1-3-9-11-8(7-19-9)15-12(18)16-11;3-2(4,5)1(6)7/h8-9,11H,1-7,13H2,(H,14,17)(H2,15,16,18);(H,6,7)/t8-,9-,11-;/m0./s1

InChI key

IVRQHQHWTOYIDN-GRIHUTHFSA-N

Application

Nucleophilic biotinylation reagent for the conjugation of various substrates and their identification and quantification using biotin-binding fluorescent labels

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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R K Garlick et al.
The Journal of biological chemistry, 263(1), 210-215 (1988-01-05)
Three N-acyl derivatives of biotinylethylenediamine were prepared: I, biotinylamidoethyl-3-(3-[125I]iodo-4-hydroxyphenyl)propionamide; II, biotinylamidoethyl-[3H]acetamide; and III, biotinylamidoethyl-3-(3,5-[125I]diiodo-4-hydroxyphenyl)propionamid e. Each compound was combined with a large excess of avidin, yielding 1:1 molar complexes. Aside from a small fraction of each complex that dissociated more

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