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Merck
CN

08124

Isoamylase from Pseudomonas sp.

ammonium sulfate suspension, ≥10,000,000 units/mg protein

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.2.1.68 (BRENDA, IUBMB)
MDL number:
MFCD00131418

Key Documents

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Product Name

Isoamylase from Pseudomonas sp., ammonium sulfate suspension, ≥10,000,000 units/mg protein

form

ammonium sulfate suspension

specific activity

≥10,000,000 units/mg protein

storage temp.

2-8°C

Quality Level

100

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Analysis Note

≥10 MU/mg protein

Application

Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.

Biochem/physiol Actions

Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.
Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Other Notes

One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)

pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN7550
BCCM9130
BCCL1638
BCCL5940
BCCM0211

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Enzymatic conversions of starch
Tomasik P and Horton D
Advances in Carbohydrate Chemistry and Biochemistry, 68, 59-436 (2012)
Digestion properties of corn starch modified by $\alpha$-D-glucan branching enzyme and cyclodextrin glycosyltransferase
Li Y, et al.
Food Hydrocolloids, 89, 534-541 (2019)
In vitro amylase hydrolysis of amylopectins from cereal starches based on molecular structure of amylopectins
Guo Li
Food Hydrocolloids, 77, 238-247 (2018)
Wenwen Li et al.
Carbohydrate polymers, 270, 118363-118363 (2021-08-09)
Finding an efficient and eco-friendly solution for starch dissolution has attracted considerable attentions in recent years. This study investigated the structural characteristics, and degradation behavior of corn starch in KOH/thiourea aqueous solution by the comparison with DMSO/LiBr and 1-allyl-3-methylimidazolium chloride
Lucio Gullo et al.
Pancreas, 38(4), 396-400 (2009-03-20)
To determine the type and frequency of pancreatic lesions detected by magnetic resonance cholangiopancreatography (MRCP) in subjects with asymptomatic pancreatic hyperenzymemia and to assess for a possible relationship between these lesions and the hyperenzymemia. From January 2005 to May 2008
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