08124
Isoamylase from Pseudomonas sp.
ammonium sulfate suspension, ≥10,000,000 units/mg protein
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MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
form
ammonium sulfate suspension
Quality Level
specific activity
≥10,000,000 units/mg protein
storage temp.
2-8°C
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Related Categories
Application
Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.
Biochem/physiol Actions
Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.
Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Unit Definition
One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)
Analysis Note
≥10 MU/mg protein
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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In vitro amylase hydrolysis of amylopectins from cereal starches based on molecular structure of amylopectins
Guo Li
Food Hydrocolloids, 77, 238-247 (2018)
Digestion properties of corn starch modified by $\alpha$-D-glucan branching enzyme and cyclodextrin glycosyltransferase
Li Y, et al.
Food Hydrocolloids, 89, 534-541 (2019)
Enzymatic conversions of starch
Tomasik P and Horton D
Advances in Carbohydrate Chemistry and Biochemistry, 68, 59-436 (2012)
Hongjie An et al.
Journal of food science, 76(1), N11-N14 (2011-05-04)
Starch debranching is fundamental for understanding the structure-function relationships of starch. In this paper, atomic force microscopy (AFM) was used to investigate potato starch by isoamylase [EC 3.2.1.68] debranching at nanometer scale. The hydrolysates were separated by gel-permeation chromatography and
Rupendra Mukerjea et al.
Carbohydrate research, 345(3), 449-451 (2009-12-26)
Amylose, a putative linear alpha-(1-->4)-glucan and a component of most starches, was isolated from potato, rice, and wheat starches by forming the 1-butanol complex in a solution of the starches. It previously had been found that these amyloses were incompletely
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