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06699

Sigma-Aldrich

Atto 532

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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50 μG
CN¥1,242.53
250 μG
CN¥4,389.46

CN¥1,242.53

Estimated to ship onJune 12, 2025Details

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50 μG
CN¥1,242.53
250 μG
CN¥4,389.46

About This Item

MDL number:
MFCD05865400
UNSPSC Code:
12352108
NACRES:
NA.25

CN¥1,242.53

Estimated to ship onJune 12, 2025Details

Request a Bulk Order

product line

BioReagent

Assay

≥90% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

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Poly(sodium 4-styrenesulfonate) solution average Mw ~1,000,000, 25 wt. % in H2O

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form

powder or solid

form

-

form

-

form

-

density

0.801 g/mL at 25 °C (lit.)

density

1.1471 g/mL at 25 °C

density

1.158 g/mL at 25 °C

density

1.163 g/mL at 25 °C

mp

460 °C (dec.)

mp

-

mp

-

mp

-

mol wt

average Mw ~70,000

mol wt

average Mw ~200,000

mol wt

average Mw ~70,000

mol wt

average Mw ~1,000,000

solubility

water and lower glycols: soluble

solubility

-

solubility

-

solubility

-

General description

Atto 532 is a new label with high molecular absorption (115,000) and quantum yield (0.90) as well as sufficient Stoke′s shift between excitation and emission maximum. It is optimized for excitation with frequency doubled Nd:YAG-Laser, and is characterized by high photostability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 532 has been conjugated with the secondary antibodies for STED (stimulated emission depletion) microscopy and immunofluorescence studies.[1][2]

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    Lot/Batch Number
    BCCL2775
    BCCK7929
    BCCK3317
    BCCK0534
    BCCJ5451

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    3D reconstruction of high-resolution STED microscope images.
    Punge A et al.
    Microscopy Research and Technique, 71, 644-644 (2008)
    Uffe V Schneider et al.
    BMC biotechnology, 10, 4-4 (2010-01-28)
    Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV
    SERRS-based detection of dye-labeled DNA using positively-charged Ag nanoparticles.
    Gill, R. and Lucassen, G. W.
    Analytical Methods : Advancing Methods and Applications, 2, 445-447 (2010)
    Daniel Aquino et al.
    Nature methods, 8(4), 353-359 (2011-03-15)
    We demonstrate three-dimensional (3D) super-resolution imaging of stochastically switched fluorophores distributed across whole cells. By evaluating the higher moments of the diffraction spot provided by a 4Pi detection scheme, single markers can be simultaneously localized with <10 nm precision in
    Stefan Bretschneider et al.
    Physical review letters, 98(21), 218103-218103 (2007-08-07)
    We report the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by transiently shelving the fluorophore in a metastable dark state. Using a relatively modest light intensity of several kW/cm(2) in a focal distribution featuring a local zero
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