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03899

Sigma-Aldrich

Prussian blue soluble

for microscopy

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About This Item

Empirical Formula (Hill Notation):
C6Fe2KN6 · xH2O
CAS Number:
Molecular Weight:
306.89 (anhydrous basis)
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy

form

powder

solubility

water: 0.1 g/10 mL, blue to very deep blue

εmax

≥120 at 253-263 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[K+].[Fe++].N#C[Fe-3](C#N)(C#N)(C#N)(C#N)C#N
[K+].[Fe++].N#C[Fe-3](C#N)(C#N)(C#N)(C#N)C#N

InChI

1S/6CN.2Fe.K/c6*1-2;;;/q;;;;;;-3;+2;+1

InChI key

ZJSIHHBKYVAMOE-UHFFFAOYSA-N

Application

Prussian blue is not a dye but an inorganic pigment containing ferric ferrocyanide. Procedures have been published for the demonstration of non-hemoglobin, cytoplasmic iron, according to Sundberg and Broman, and for the demonstration of hemosiderin according to Highman and from Lille and Fullmer based the work originally done by Perle in 1867. Prussian Blue is formed in situ in both of these methods. In combination with iron particles such as superparamagnetic iron oxide nanoparticles, Prussian blue may be used for tracking of transplanted cells such as mesenchymal stem cells. Prussian blue may also be used to differentiate slow versus fast cycling cells by methods that monitor iron oxide particle labeling dilution thru cell division.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The application of the Prussian blue stain to previously stained films of blood and bone marrow.
R D SUNDBERG et al.
Blood, 10(2), 160-166 (1955-02-01)
In vivo and ex vivo MR imaging of slowly cycling melanoma cells.
Magnitsky S, Roesch A, Herlyn M, Glickson JD.
Magn. Reson. in Med., 66, 1362-1373 (2011)
Gerlinde Schmidtke-Schrezenmeier et al.
Cytotherapy, 13(8), 962-975 (2011-04-16)
Mesenchymal stromal cells (MSC) are the focus of research in regenerative medicine aiming at the regulatory approval of these cells for specific indications. To cope with the regulatory requirements for somatic cell therapy, novel approaches that do not interfere with
Hiroshi Koiwaya et al.
Journal of molecular and cellular cardiology, 51(1), 33-40 (2011-03-31)
Augmenting neovascularization with the use of endothelial progenitor cells (EPCs) is a therapeutic option to rescue critical limb ischemia (CLI). However, the outcomes have been not so satisfactory. The detectable number of injected EPCs at the ischemic site is rather
Nachweis von eisenoxyd in Gewissen pigmenten
Virchow?s Arch. (Pathol. Anat.), 39, 42-48 (1867)

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