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Key Documents

Safety Information

03722

Sigma-Aldrich

Atto 580 Q

BioReagent

Synonym(s):

Atto 580 quencher, Atto Q 580

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About This Item

MDL number:
UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

manufacturer/tradename

ATTO-TEC GmbH

technique(s)

UV/Vis spectroscopy: suitable

solubility

DMF: soluble
DMSO: soluble

suitability

corresponds for UV test

storage temp.

−20°C

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This Item
OGS636OGS635OGS632
peptide cleavage

EKT

peptide cleavage

EKT

peptide cleavage

EKT

peptide cleavage

EKT

peptide tag location

N-terminal

peptide tag location

N-terminal

peptide tag location

C-terminal

peptide tag location

N-terminal

origin of replication

pUC

origin of replication

pUC

origin of replication

pUC

origin of replication

pUC

promoter

Promoter name: Tac
Promoter activity: inducible
Promoter type: bacterial

promoter

Promoter name: T7 Lac
Promoter activity: inducible
Promoter type: bacterial

promoter

Promoter name: Tac
Promoter activity: inducible
Promoter type: bacterial

promoter

Promoter name: Tac
Promoter activity: inducible
Promoter type: bacterial

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

General description

Atto 580Q is a new quencher for fluorescence characterized by high molecular absorption (110.000) and an absorption maximum at 586 nm (H2O). Atto 580Q is characterized by a high photostability and thermostability. It is moderately hydrophilic, and soluble in polar solvents as DMF or DMSO.

Application

Ato 580Q can be utilized as a fluorescence quencher (λ absorbance = 586 nm) on amine-labeled nucleotides for FRET experiments. Works well in combination with the following fluorescent dyes: TAMRA, ATTO 550, ATTO 565, ATTO 590, and ROX.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Articles

    Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

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