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Sigma-Aldrich

Atto 495 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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MDL number:
MFCD06798157
UNSPSC Code:
12352108
PubChem Substance ID:
329747054
NACRES:
NA.32

product line

BioReagent

Quality Level

100

Assay

≥90% (HPLC)
≥90% (degree of coupling)

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble
DMSO: soluble
acetonitrile: soluble

λ

in acetonitrile (with 0.1% acetic acid)

UV absorption

λ: 496-502 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[O-]Cl(=O)(=O)=O.CN(C)c1ccc2C=C3C=C\C(C=C3N(CCCC(=O)ON4C(=O)CCC4=O)c2c1)=[N+](\C)C

InChI

1S/C25H29N4O4.ClHO4/c1-26(2)19-9-7-17-14-18-8-10-20(27(3)4)16-22(18)28(21(17)15-19)13-5-6-25(32)33-29-23(30)11-12-24(29)31;2-1(3,4)5/h7-10,14-16H,5-6,11-13H2,1-4H3;(H,2,3,4,5)/q+1;/p-1

InChI key

QYNYOHCMTHAQIE-UHFFFAOYSA-M

Application

Atto 495 is a new label for proteins and oligo-nucleotides with high molecular absorption (80.000) and quantum yield (0.45) as well as sufficient stoke′s shift (excitation maximum 495 nm, emission maximum 527 nm). Atto 495 is characterized by a high photostability and thermostability. It is slightly hydrophilic, and solubility in polar solvents as DMF or DMSO is high.
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Other Notes

Incorporation in a zeolite photonic nanodevice

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H. Maas, G. Calzaferri
The Spectrum, 16, 18-18 (2003)
Adolfo Téllez et al.
Electrophoresis, 29(18), 3916-3923 (2008-10-14)
Three equilibria determine the interaction of a neutral analyte with the detergent in micellar electrokinetic chromatography and therefore its migration: (i) that of the free analyte in the aqueous phase with the micelle, (ii) its association with free detergent monomers
Amos Bardea et al.
Analytical chemistry, 83(24), 9418-9423 (2011-11-02)
We report on a new ultrasensitive and fast technique for the detection and identification of both DNA and RNA with sensitivity of a few molecules. The new method is based on a patterned capillary tube (PCT) in which the internal
Verena Schoewel et al.
PloS one, 7(11), e49603-e49603 (2012-11-28)
Mutations in the dysferlin gene cause the most frequent adult-onset limb girdle muscular dystrophy, LGMD2B. There is no therapy. Dysferlin is a membrane protein comprised of seven, beta-sheet enriched, C2 domains and is involved in Ca(2+)dependent sarcolemmal repair after minute
Andreas Greth et al.
PloS one, 7(6), e38999-e38999 (2012-06-23)
The blood stage of the plasmodium parasite life cycle is responsible for the clinical symptoms of malaria. Epidemiological studies have identified coincidental malarial endemicity and multiple red blood cell (RBC) disorders. Many RBC disorders result from mutations in genes encoding
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