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Merck
CN

00379

Atto 495 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

UNSPSC Code:
12352108
PubChem Substance ID:
329747054
NACRES:
NA.32
MDL number:
MFCD06798157

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Product Name

Atto 495 NHS ester, BioReagent, suitable for fluorescence, ≥90% (HPLC)

SMILES string

[O-]Cl(=O)(=O)=O.CN(C)c1ccc2C=C3C=C\C(C=C3N(CCCC(=O)ON4C(=O)CCC4=O)c2c1)=[N+](\C)C
[O-]Cl(=O)(=O)=O.CN(C)c1ccc2C=C3C=C\C(C=C3N(CCCC(=O)ON4C(=O)CCC4=O)c2c1)=[N+](\C)C

InChI

1S/C25H29N4O4.ClHO4/c1-26(2)19-9-7-17-14-18-8-10-20(27(3)4)16-22(18)28(21(17)15-19)13-5-6-25(32)33-29-23(30)11-12-24(29)31;2-1(3,4)5/h7-10,14-16H,5-6,11-13H2,1-4H3;(H,2,3,4,5)/q+1;/p-1

InChI key

QYNYOHCMTHAQIE-UHFFFAOYSA-M

product line

BioReagent

assay

≥90% (HPLC)
≥90% (degree of coupling)

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble
DMSO: soluble
acetonitrile: soluble

λ

in acetonitrile (with 0.1% acetic acid)

UV absorption

λ: 496-502 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

Application

Atto 495 is a new label for proteins and oligo-nucleotides with high molecular absorption (80.000) and quantum yield (0.45) as well as sufficient stoke′s shift (excitation maximum 495 nm, emission maximum 527 nm). Atto 495 is characterized by a high photostability and thermostability. It is slightly hydrophilic, and solubility in polar solvents as DMF or DMSO is high.
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Other Notes

Incorporation in a zeolite photonic nanodevice

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP0729
BCCN7130
BCCN0569
BCCL4347
BCCL2771

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