C2432
Chloroform
contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Synonym(s):
Methylidyne trichloride, Trichloromethane
About This Item
Recommended Products
vapor density
4.1 (vs air)
Quality Level
vapor pressure
160 mmHg ( 20 °C)
Assay
≥99.5%
form
liquid
contains
100-200 ppm amylenes as stabilizer
technique(s)
RNA extraction: suitable
refractive index
n20/D 1.445 (lit.)
bp
60.5-61.5 °C (lit.)
mp
−63 °C (lit.)
density
1.492 g/mL at 25 °C (lit.)
SMILES string
ClC(Cl)Cl
InChI
1S/CHCl3/c2-1(3)4/h1H
InChI key
HEDRZPFGACZZDS-UHFFFAOYSA-N
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General description
Application
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
Target Organs
Central nervous system, Liver,Kidney
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What amount of amylenes are present in Product C2432, Chloroform, as a stabilizer?
The concentration of amylenes is 100 - 300 ppm.
Has Product C2432, Chloroform, been tested for DNase and RNase activity?
This product has not been tested for DNase and RNase activity. However, it has been tested and found to be suitable for the extraction of nucleic acids based upon the evaluation of extracted DNA by agarose electrophoresis.
How can Product C2432, Chloroform, be used to extract proteins from nucleic acids?
A protocol for using phenol:chloroform or phenol:chloroform:isoamyl alcohol to extract proteins from nucleic acids is described in Molecular Cloning: A Laboratory Manual, vol. 3, pp E.3 - E.4 (1989).
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