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60220

Sigma-Aldrich

Potassium phosphate monobasic

buffer substance, anhydrous, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99.5-100.5%

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Synonym(s):
Monopotassium phosphate, Potassium dihydrogen phosphate, prim.-Potassium phosphate
Linear Formula:
KH2PO4
CAS Number:
Molecular Weight:
136.09
EC Number:
MDL number:
UNSPSC Code:
12352300
PubChem Substance ID:
NACRES:
NA.21

grade

ACS reagent
anhydrous
puriss. p.a.

Quality Level

Agency

reag. ISO
reag. Ph. Eur.

Assay

99.5-100.5%

form

crystalline

impurities

≤0.001% total nitrogen (N)

loss

≤0.2% loss on drying, 105 °C

pH

4.2-4.5 (20 °C, 5%)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

mp

252.6 °C (lit.)

solubility

water: 0.5 g/10 mL, clear, colorless to light yellow

density

2.338 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

As: ≤0.5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤15 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg
heavy metals: ≤0.001% (by ICP)

SMILES string

[K+].OP(O)([O-])=O

InChI

1S/K.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

GNSKLFRGEWLPPA-UHFFFAOYSA-M

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General description

Potassium phosphate is widely used in buffers to prepare biological samples and examine biological interactions.

Application

Potassium phosphate monobasic has been used:
  • to prepare the working luminol solution to study the prooxidant activity in the chemiluminescent system luminol–H2O2
  • in phosphate buffer saline (PBS) to culture embryonic stem cells (ESCs) and generate embryoid bodies
  • in PBS to prepare polydimethylsiloxane (PDMS) substrate

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Investigation on the effects of disease-associated mutations on neurodevelopment is an essential approach to understand the molecular basis of neurological disorders and can be achieved by generating suitable animal models. However, some of the mutations preclude development of animal models
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The purpose of this study was to examine whether speed endurance training (SET, repeated 30-s sprints) and heavy resistance training (HRT, 80-90% of 1 repetition maximum) performed in succession are compatible and lead to performance improvements in moderately trained endurance

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