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Merck
CN

270733

Water

suitable for HPLC

Synonym(s):

Aqua, Dihydrogen oxide

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About This Item

Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
UNSPSC Code:
12190000
NACRES:
NA.21
PubChem Substance ID:
329752968
EC Number:
231-791-2
Beilstein/REAXYS Number:
2050024
MDL number:
MFCD00011332
Grade:
HPLC grade
Technique(s):
HPLC: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg

Key Documents

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Product Name

Water, suitable for HPLC

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

InChI

1S/H2O/h1H2

SMILES string

O

grade

HPLC grade

vapor density

<1 (vs air)

vapor pressure

3 mmHg

form

liquid

technique(s)

HPLC: suitable

evapn. residue

<0.0003%

refractive index

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

λ

H2O reference

UV absorption

λ: 205 nm Amax: 0.01
λ: 210 nm Amax: 0.01
λ: 250-400 nm Amax: 0.005

Quality Level

200

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Application

Water is used as a:
  • Solvent to prepare buffers during the cell lysis process.
  • Component of the mobile phase during the HPLC–UV–diode-array detection for the separation and detection of the analytes.

General description

Water suitable for HPLC is commonly used as a solvent for isocratic elution in both cation and anion chromatography. Additionally, it is used as a component to prepare standard stock solutions, and for evaporation under a nitrogen stream in a water bath to concentrate the sample before LC-MS-MS analysis.

Other Notes

Important notice
The article number 270733-6X1L will be discontinued. Please order the single bottle 270733-1L which is physically identical with the same exact specifications.
Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Packaging

M-Bottle for Solvents
As a global leader in lab reagents, we are constantly looking for new ways to optimize the safety of our products. The newly developed 4L solvent bottle design features advanced sealing technology that eliminates leaks to make the handling of solvents safer and more convenient than ever before.
See all the new features here!

Preparation Note

Product filtered through a 0.2 μm filter

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT4414
BCCP0259
BCCN9934
BCCN9737
BCCN9469

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Rapid confirmatory assay for the simultaneous detection of ronidazole, metronidazole and dimetridazole in eggs using liquid chromatography-tandem mass spectrometry
Els D et al.
Analyst, 125, 1533-1535 (2000)
Identification of the cysteine protease legumain as a potential chronic hypoxia-specific multiple myeloma target gene
Ada-Sophia C et al.
Cells, 11, 292-292 (2022)
Milk ultrafiltrate analysis by ion chromatography and calcium activity for SMUF preparation for different scientific purposes and prediction of its supersaturation
Joseph D et al.
International dairy journal, 68, 60-69 (2017)
Optimization and validation of HPLC-UV-DAD and HPLC-APCI-MS methodologies for the determination of selected PAHs in water samples
Titato, Guilherme T M et al.
Journal of Chromatographic Science, 44, 35-40 (2006)
Manolis Mandalakis et al.
Journal of chromatography. A, 1217(1), 143-150 (2009-12-08)
The analysis of amino acids by gas chromatography mass spectrometry (GC-MS) after their derivatization with N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide was investigated as an alternative approach for the determination of free (FAA) and combined amino acids (CAA) in aerosols. This technique showed excellent linearity
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