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Merck
CN

T8533

o-Tolidine

≥95% purity, powder or chunks

Synonym(s):

3,3′-Dimethylbenzidine, 4,4′-Bianisidine

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About This Item

Linear Formula:
[-C6H3(CH3)-4-NH2]2
CAS Number:
119-93-7
Molecular Weight:
212.29
NACRES:
NA.47
PubChem Substance ID:
24900539
UNSPSC Code:
12171500
EC Number:
204-358-0
MDL number:
MFCD00014773
Beilstein/REAXYS Number:
2210640

Key Documents

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Product Name

o-Tolidine, ≥95%

SMILES string

Cc1cc(ccc1N)-c2ccc(N)c(C)c2

InChI

1S/C14H16N2/c1-9-7-11(3-5-13(9)15)12-4-6-14(16)10(2)8-12/h3-8H,15-16H2,1-2H3

InChI key

NUIURNJTPRWVAP-UHFFFAOYSA-N

assay

≥95%

form

powder or chunks

technique(s)

titration: suitable

color

white to light brown

mp

128-131 °C (lit.)

solubility

water: slightly soluble 1.3 g/L at 25 °C

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

o-Tolidine has also been used to determine the presence of laccase activity in T. versicolor fungal colonies.
Sensitive colorimetric reagent for gold and for free chlorine in water.

Disclaimer

May be carcinogenic.

General description

o-Tolidine is a diacidic dye which is sparingly soluble in water and easily soluble in ether and alcohol. On exposure to chlorine or bromine vapors, it forms an intense blue color. This forms the basis for detection of chlorine, nitrates and hydrogen peroxide in air or water. It turns yellow on reacting with free chlorine. o-Tolidine easily forms acid salts.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

212.0 °F - closed cup

flash_point_c

100 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ0060
MKCX1219
MKCV7349
MKCS9398
MKCT8349

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Purification and Characterization of Laccase from the
White Rot Fungus Trametes versicolor
Moon-Jeong
Journal of Microbiology (2005)
Wiley-VCH
Ullmann's Fine Chemicals null
Leo M.L. Nollet
Handbook of Water Analysis (2013)
S P Tuffli et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 13(2), 177-179 (2001-04-06)
Eight different diets were each fed to 6 cats to evaluate the effect on a guaiac and an o-tolidine fecal occult blood test. Fecal samples were collected from day 5 through day 7. Canine blood or pure cottage cheese were
V V Sentchouk et al.
Biochemistry. Biokhimiia, 69(2), 201-207 (2004-03-06)
Human thyroid peroxidase (hTPO) catalyzes a one-electron oxidation of benzidine derivatives by hydrogen peroxide through classical Chance mechanism. The complete reduction of peroxidase oxidation products by ascorbic acid with the regeneration of primary aminobiphenyls was observed only in the case
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