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Safety Information

T8533

Sigma-Aldrich

o-Tolidine

≥95% purity, powder or chunks

Synonym(s):

3,3′-Dimethylbenzidine, 4,4′-Bianisidine

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About This Item

Linear Formula:
[-C6H3(CH3)-4-NH2]2
CAS Number:
119-93-7
Molecular Weight:
212.29
Beilstein:
2210640
EC Number:
204-358-0
MDL number:
MFCD00014773
UNSPSC Code:
12171500
PubChem Substance ID:
24900539
NACRES:
NA.47

Product Name

o-Tolidine, ≥95%

Quality Level

200

Assay

≥95%

form

powder or chunks

technique(s)

titration: suitable

color

white to light brown

mp

128-131 °C (lit.)

solubility

water: slightly soluble 1.3 g/L at 25 °C

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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General description

o-Tolidine is a diacidic dye which is sparingly soluble in water and easily soluble in ether and alcohol. On exposure to chlorine or bromine vapors, it forms an intense blue color. This forms the basis for detection of chlorine, nitrates and hydrogen peroxide in air or water. It turns yellow on reacting with free chlorine. o-Tolidine easily forms acid salts.

Application

o-Tolidine has also been used to determine the presence of laccase activity in T. versicolor fungal colonies.
Sensitive colorimetric reagent for gold and for free chlorine in water.

Warning

May be carcinogenic.

Signal Word

Danger

Hazard Statements

H302,H350,H411

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

212.0 °F - closed cup

Flash Point(C)

100 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

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Certificates of Analysis (COA)

Lot/Batch Number
MKCX1219
MKCV7349
MKCS9398
MKCT8349
MKCR8513

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Purification and Characterization of Laccase from the
White Rot Fungus Trametes versicolor
Moon-Jeong
Journal of Microbiology (2005)
Wiley-VCH
Ullmann's Fine Chemicals null
Leo M.L. Nollet
Handbook of Water Analysis (2013)
3,3'-Dimethylbenzidine.
Report on carcinogens : carcinogen profiles, 10, 104-105 (2004-08-25)
Zhijun Cao et al.
Biosensors & bioelectronics, 23(3), 348-354 (2007-06-06)
The electrooxidation of o-tolidine (oTD) was investigated via the electrochemical quartz crystal microbalance (EQCM) technique. The formation and breakage of the poorly soluble charge-transfer complex (CTC) occurred during the redox switching of oTD, and the CTC precipitation on and its
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