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T8533

Sigma-Aldrich

o-Tolidine

≥95% purity, powder or chunks

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Synonym(s):
3,3′-Dimethylbenzidine, 4,4′-Bianisidine
Linear Formula:
[-C6H3(CH3)-4-NH2]2
CAS Number:
119-93-7
Molecular Weight:
212.29
Beilstein:
2210640
EC Number:
204-358-0
MDL number:
MFCD00014773
UNSPSC Code:
12171500
PubChem Substance ID:
24900539
NACRES:
NA.47

Quality Level

200

Assay

≥95%

form

powder or chunks

color

white to light brown

mp

128-131 °C (lit.)

solubility

water: slightly soluble 1.3 g/L at 25 °C

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(ccc1N)-c2ccc(N)c(C)c2

InChI

1S/C14H16N2/c1-9-7-11(3-5-13(9)15)12-4-6-14(16)10(2)8-12/h3-8H,15-16H2,1-2H3

InChI key

NUIURNJTPRWVAP-UHFFFAOYSA-N

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General description

o-Tolidine is a diacidic dye which is sparingly soluble in water and easily soluble in ether and alcohol. On exposure to chlorine or bromine vapors, it forms an intense blue color. This forms the basis for detection of chlorine, nitrates and hydrogen peroxide in air or water. It turns yellow on reacting with free chlorine. o-Tolidine easily forms acid salts.

Application

o-Tolidine has also been used to determine the presence of laccase activity in T. versicolor fungal colonies.
Sensitive colorimetric reagent for gold and for free chlorine in water.

Warning

May be carcinogenic.

Signal Word

Danger

Hazard Statements

H302,H350,H411

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B

WGK

WGK 3

Flash Point(F)

closed cup

Flash Point(C)

closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wiley-VCH
Ullmann's Fine Chemicals null
Purification and Characterization of Laccase from the
White Rot Fungus Trametes versicolor
Moon-Jeong
Journal of Microbiology (2005)
3,3'-Dimethylbenzidine.
Report on carcinogens : carcinogen profiles, 10, 104-105 (2004-08-25)
Zhijun Cao et al.
Biosensors & bioelectronics, 23(3), 348-354 (2007-06-06)
The electrooxidation of o-tolidine (oTD) was investigated via the electrochemical quartz crystal microbalance (EQCM) technique. The formation and breakage of the poorly soluble charge-transfer complex (CTC) occurred during the redox switching of oTD, and the CTC precipitation on and its
3,3'-Dimethylbenzidine and dyes metabolized to 3,3'-dimethylbenzidine: 3,3'-dimethylbenzidine.
Report on carcinogens : carcinogen profiles, 12, 168-170 (2011-08-20)
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