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T17917

Sigma-Aldrich

Tricine

≥99% (titration), reagent grade, crystalline

Synonym(s):

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
5704-04-1
Molecular Weight:
179.17
Beilstein:
1937804
MDL number:
MFCD00004277
UNSPSC Code:
12161700
NACRES:
NA.25

biological source

synthetic

Quality Level

200

grade

reagent grade

Assay

≥99% (titration)

form

crystalline

storage condition

(Tightly closed. Dry)

impurities

<0.5% water

color

white

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

solubility

water: 0.1 M, clear, colorless

cation traces

Pb: ≤5 ppm

storage temp.

room temp

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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General description

Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.

Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.

Application

Tricine is intended to use for:
  • Electrophoresis To separate low-weight peptides and proteins
  • Crystallization of proteins of varying pH
  • Resuspension of cell pellets
  • Enzyme assays
  • Tissue culture
Buffer component for separation of low molecular weight peptides.

Features and Benefits

  • Ideal as a Biological buffer for Cell Biology and Biochemical research
  • Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)

Other Notes

For additional information on our range of Biochemicals, please complete this form.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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H Schägger et al.
Analytical biochemistry, 166(2), 368-379 (1987-11-01)
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in

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