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Merck
CN

T0138

Tetrazolium Violet

≥98% purity (TLC), powder

Synonym(s):

2,5-Diphenyl-3-(α-naphthyl)tetrazolium chloride, 2,5-Diphenyl-3-(1-naphthyl)tetrazolium chloride, TV

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About This Item

Empirical Formula (Hill Notation):
C23H17ClN4
CAS Number:
1719-71-7
Molecular Weight:
384.86
NACRES:
NA.47
PubChem Substance ID:
24899868
UNSPSC Code:
12171500
EC Number:
217-008-7
MDL number:
MFCD00011875

Key Documents

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Product Name

Tetrazolium Violet,

InChI key

RONADMZTCCPLEF-UHFFFAOYSA-M

InChI

1S/C23H17N4.ClH/c1-3-11-19(12-4-1)23-24-26(20-14-5-2-6-15-20)27(25-23)22-17-9-13-18-10-7-8-16-21(18)22;/h1-17H;1H/q+1;/p-1

SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4cccc5ccccc45

assay

≥98% (TLC)

form

powder

mp

247-249 °C

solubility

methanol: 1 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

General description

Tetrazolium Violet is a monotetrazolium salt which forms water-insoluble formazans. These salts are mainly used as indicators of extracellular redox activity and cell redox potential. Tetrazolium violet is positively charged and cell-permeable.

Application

Tetrazolium Violet has been used to stain agar layers in bacteriophage plaque formation assays.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCF7875
MKCC6990
MKBZ1891V
MKBV9011V
MKBT2079V

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Differential Effect of Tetrazolium Dyes upon Bacteriophage Plaque Assay Titers
CHRISTON J. HURST
Applied and Environmental Microbiology, 3462-3465 (1994)
Experimental conditions affect the site of tetrazolium violet reduction in the electron transport chain of Lactococcus lactis
Sybille Tachon
Microbiology, 2941-2948 (2009)
Dongsheng Zhang et al.
Journal of virology, 89(2), 1419-1427 (2014-11-14)
Tulane virus (TV), the prototype of the Recovirus genus in the calicivirus family, was isolated from the stools of rhesus monkeys and can be cultivated in vitro in monkey kidney cells. TV is genetically closely related to the genus Norovirus
Robert B Lull et al.
Psychological bulletin, 141(5), 1022-1048 (2015-07-21)
It is commonly assumed that sex and violence sell. However, we predicted that sex and violence would have the opposite effect. We based our predictions on the evolution and emotional arousal theoretical framework, which states that people are evolutionarily predisposed
R Jugdaohsingh et al.
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 26(4), 1405-1415 (2015-01-13)
Observational (epidemiological) studies suggest the positive association between dietary silicon intake and bone mineral density may be mediated by circulating estradiol level. Here, we report the results of a silicon supplementation study in rats that strongly support these observations and
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