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T0138

Sigma-Aldrich

Tetrazolium Violet

≥98% purity (TLC), powder

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Synonym(s):
2,5-Diphenyl-3-(α-naphthyl)tetrazolium chloride, 2,5-Diphenyl-3-(1-naphthyl)tetrazolium chloride, TV
Empirical Formula (Hill Notation):
C23H17ClN4
CAS Number:
1719-71-7
Molecular Weight:
384.86
EC Number:
217-008-7
MDL number:
MFCD00011875
UNSPSC Code:
12171500
PubChem Substance ID:
24899868
NACRES:
NA.47

Assay

≥98% (TLC)

Quality Level

200

form

powder

mp

247-249 °C

solubility

methanol: 1 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4cccc5ccccc45

InChI

1S/C23H17N4.ClH/c1-3-11-19(12-4-1)23-24-26(20-14-5-2-6-15-20)27(25-23)22-17-9-13-18-10-7-8-16-21(18)22;/h1-17H;1H/q+1;/p-1

InChI key

RONADMZTCCPLEF-UHFFFAOYSA-M

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General description

Tetrazolium Violet is a monotetrazolium salt which forms water-insoluble formazans. These salts are mainly used as indicators of extracellular redox activity and cell redox potential. Tetrazolium violet is positively charged and cell-permeable.

Application

Tetrazolium Violet has been used to stain agar layers in bacteriophage plaque formation assays.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differential Effect of Tetrazolium Dyes upon Bacteriophage Plaque Assay Titers
CHRISTON J. HURST
Applied and Environmental Microbiology, 3462-3465 (1994)
Experimental conditions affect the site of tetrazolium violet reduction in the electron transport chain of Lactococcus lactis
Sybille Tachon
Microbiology, 2941-2948 (2009)
Laura Turner et al.
PloS one, 10(7), e0133972-e0133972 (2015-07-30)
Children's exposure to food marketing is one environmental determinant of childhood obesity. Measuring the extent to which children are aware of food brands may be one way to estimate relative prior exposures to food marketing. This study aimed to develop
Dongsheng Zhang et al.
Journal of virology, 89(2), 1419-1427 (2014-11-14)
Tulane virus (TV), the prototype of the Recovirus genus in the calicivirus family, was isolated from the stools of rhesus monkeys and can be cultivated in vitro in monkey kidney cells. TV is genetically closely related to the genus Norovirus
Huijing Shi et al.
Journal of epidemiology, 25(9), 574-582 (2015-07-28)
Phthalates are synthetic chemicals and ubiquitous environmental contaminants, with hormonal activity that may alter the course of pubertal development in children. To determine whether exposure to phthalate metabolites is associated with timing of pubertal development in a cross-sectional study of
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