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Key Documents

S7907

Sigma-Aldrich

Sodium phosphate dibasic

BioXtra, ≥99.0%

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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3 EA
CN¥3,218.14

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Estimated to ship onApril 28, 2025Details


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3 EA
CN¥3,218.14

About This Item

Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
EC Number:
MDL number:
UNSPSC Code:
12161700
eCl@ss:
38070211
PubChem Substance ID:
NACRES:
NA.25

CN¥3,218.14


Estimated to ship onApril 28, 2025Details


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product line

BioXtra

Quality Level

Assay

≥99.0%

form

powder or crystals

impurities

≤0.1% Insoluble matter

pH

8.0-11.0 (20 °C, 1 M in H2O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.002%
Cu: ≤0.0005%
Fe: ≤0.002%
K: ≤0.005%
Mg: ≤0.005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

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General description

Sodium phosphate dibasic (NaH2PO4), also known as disodium phosphate, is a commonly used phosphate salt in biochemistry research due to its versatile properties. It is a key component in the preparation of phosphate buffer saline (PBS)[1] and phosphate buffer, a commonly used buffer solution in many biological applications. In addition to its buffer properties, NaH2PO4 is also used as a precipitating agent to form silver orthophosphate (Ag3PO4) powders,[2] making it a valuable tool in many laboratory settings. Disodium phosphate is also a widely used emulsifier in dairy products such as cheese and other foods.[3]

Application

[4]Sodium phosphate dibasic has been used:
  • as a component of phosphate buffered saline (PBS)[1]
  • as a component of buffer for the preparation of cryopreservation diluent[4]
  • in the preparation of McIlvaine buffer (citrate-phosphate buffer) for toluidine blue staining[5]
  • in the preparation of the S9 mix for the Ames test, and SOS Chromotest[6]
  • in the preparation of calcium deficient hydroxyapatite (CDHA) paste during the fabrication process of the CDHA scaffold[7]

Features and Benefits

  • The BioXtra product line ensures high-quality products for consistent results.
  • High level of assay ( ≥99.0%), indicating its purity.
  • Powder form for easy handling and use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sequential generations of honey bee (Apis mellifera) queens produced using cryopreserved semen
Hopkins BK, et al.
Reproduction, Fertility, and Development, 24(8), 1079-1083 (2012)
Effect of Emulsifier Salts on Textural and Flavor Properties of Processed Cheeses
Gupta SK, et al.
Journal of Dairy Science, 67(4), 764-778 (1984)
Sperm protamine-status correlates to the fertility of breeding bulls
Dogan S, et al.
Biology of Reproduction, 92(4) (2015)
Whole-mount Immunohistochemistry of Adult Zebrafish Retina for Advanced Imaging
Nagashima M and Hitchcock PF
Bio-protocol, 10(24) (2020)
Injectable magnesium oxychloride cement foam-derived scaffold for augmenting osteoporotic defect repair
Zhu Y, et al.
Journal of Colloid and Interface Science (2023)

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