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S7545

Sigma-Aldrich

Sodium acetate

BioXtra, ≥99.0%

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Synonym(s):
Acetic acid sodium salt
Linear Formula:
CH3COONa
CAS Number:
127-09-3
Molecular Weight:
82.03
Beilstein:
3595639
EC Number:
204-823-8
MDL number:
MFCD00012459
UNSPSC Code:
12161700
eCl@ss:
39021906
PubChem Substance ID:
24899754
NACRES:
NA.25

product line

BioXtra

Quality Level

200

Assay

≥99.0%

form

solid

autoignition temp.

1112 °F

impurities

≤0.0005% Phosphorus (P)
<0.01% Insoluble matter

pH

8.5-9.9 (25 °C, 246 g/L)

pKa 

4.76 (acetic acid)

mp

>300 °C (dec.) (lit.)

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.002%
sulfate (SO42-): ≤0.003%

cation traces

Al: ≤0.0005%
Ca: ≤0.005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.03%
Mg: ≤0.0005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

SMILES string

[Na+].CC([O-])=O

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

InChI key

VMHLLURERBWHNL-UHFFFAOYSA-M

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General description

Sodium acetate is commonly applicable in molecular biology experiments such as nucleic acid purification and precipitation, protein gel electrophoresis, protein crystallization, and high-performance liquid chromatography (HPLC). Sodium acetate is also used in plastic production, dyeing, and leather tanning.

Application

Sodium acetate has been used:
  • in the isolation of DNA from bacterial samples of the human gastrointestinal tract
  • as a component of RNA elution buffer for the preparation of infrared (IR) fluorescent probes in northern blot technique
  • in the lysis buffer for cellular lysis to detect reversible oxidation of protein tyrosine phosphatases (PTPs) with the cysteinyl-labeling assay using IMR90 fibroblasts

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Density, viscosity and ultrasonic velocity studies of aqueous solutions of sodium acetate at different temperatures
Kharat SJ
Journal of Molecular Liquids, 140(1-3), 10-14 (2008)
Christopher Fields et al.
Bio-protocol, 9(8) (2019-08-21)
Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern). Our method is as sensitive as other non-radioactive
Erwin G Zoetendal et al.
Nature protocols, 1(2), 870-873 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that develops in time and space. The most widely used approaches to study microbial diversity and activity are all based on the analysis of nucleic acids, DNA, rRNA and mRNA.
Graham R Scott et al.
Molecular biology and evolution, 32(8), 1962-1976 (2015-04-09)
At high-altitude, small mammals are faced with the energetic challenge of sustaining thermogenesis and aerobic exercise in spite of the reduced O2 availability. Under conditions of hypoxic cold stress, metabolic demands of shivering thermogenesis and locomotion may require enhancements in
Mari Endo et al.
Journal of gastroenterology, 49(6), 1026-1039 (2013-07-13)
Daikenchuto (DKT), a gastrointestinal prokinetic Japanese herbal medicine, is prescribed for patients with postoperative ileus (POI) and adhesive bowel obstruction following abdominal surgery. Several mechanisms for the amelioration of POI by DKT have been suggested; however, it has remained unclear
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